Antimicrobial and DNA-binding activities of the peptide fragments of human lactoferrin and histatin 5 against Streptococcus mutans
Antimicrobial and DNA-binding activities of the peptide fragments of human lactoferrin and histatin 5 against Streptococcus mutans
复制标题
人乳铁蛋白和组蛋白 5 的肽片段对变形链球菌的抗菌和 DNA 结合活性
DOI：
10.1016/j.archoralbio.2011.02.004
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发表时间：
2011-09-01
影响因子：
3
通讯作者：
Gu, Lisha
中科院分区：
文献类型：
--
作者：
Huo, Lijun;Zhang, Kai;Gu, Lisha
目标：为研究两种唾液抗菌肽hLF 1 11和P 113对口腔链球菌的杀灭作用，并探讨其抗菌机制，采用微量肉汤稀释法测定两种唾液抗菌肽hLF 1 11和P 113对口腔链球菌的抗菌活性。通过扫描电子显微镜观察hLF 1 -11和P-113对细菌质膜的影响。使用细胞内染料钙黄绿素监测细胞膜通透性。荧光显微镜下观察hLF 1 -11和P-113在细菌中的亚细胞定位。结果：hLF 1 - 11和P-113对口腔链球菌均有较强的杀菌活性。变形链球菌UA 159是测试的口腔细菌物种中对三种肽的抗微生物作用最敏感的。hLF 1 -11和P-113处理30 min后，细菌细胞膜仍保持完整，hLF 1 -11和P-113可穿透细菌细胞膜，在S.变种人结论：hLF 1 - 11和P-113对致龋链球菌具有抗菌活性。通过可能涉及DNA结合的细胞内机制来抑制变形杆菌。因此，这些肽可能是有吸引力的和有价值的候选人开发成有效的抗微生物疗法，以打击龋齿。(C)2011爱思唯尔有限公司版权所有。
Objective: To investigate the killing effect of two salivary antimicrobial peptides, hLF1-11 and P-113, and identify the antibacterial mechanism of the peptides.Methods: The antimicrobial activities of hLF1-11 and P-113 against oral Streptococci strains were determined using the broth microdilution method. The effects of hLF1-11 and P-113 on the bacterial plasma membrane were visualized by scanning electron microscopy. Cell membrane permeability was monitored using the intracellular dye calcein. The subcellular localization of hLF1-11 and P-113 in bacteria was measured by fluorescence light microscopy. An electrophoretic mobility shift assay (EMSA) was performed to evaluate the DNA binding capabilities of hLF1-11, P-113 and MUC7 12-mer.Results: Both hLF1-11 and P-113 exerted potent bactericidal activities against all selected oral Streptococci. Streptococcus mutans UA 159 was the most susceptible of the oral bacterial species tested to the antimicrobial effects of the three peptides. The cell membranes of bacteria treated with hLF1-11 or P-113 were still intact after 30 min. hLF1-11 and P-113 could penetrate the bacterial cell membranes and accumulate in the cytoplasm in S. mutans. Both hLF1-11 and P-113 showed DNA binding affinity.Conclusions: Together, our results demonstrate that hLF1-11 and P-113 display antibacterial activity against dental cavity-inducing S. mutans through an intracellular mechanism that could involve DNA binding. Thus, these peptides might be attractive and valuable candidates for development into effective antimicrobial therapies to combat dental caries. (C) 2011 Elsevier Ltd. All rights reserved.