Expression patterns of Wnt genes during development of an anterior part of the chicken eye
Expression patterns of Wnt genes during development of an anterior part of the chicken eye
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DOI：
10.1002/dvdy.20621
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发表时间：
2006-02-01
影响因子：
2.5
通讯作者：
Frolova, EI
中科院分区：
文献类型：
--
作者：
Fokina, VM;Frolova, EI
为了解决Wnt在前眼发育中的作用，我们使用鸡模型对前眼发育过程中所有Wnt基因进行全面的表达分析。在分析现有的基因组序列中，我们发现鸡基因组编码18个Wnt蛋白，这些蛋白与相应的人类和小鼠蛋白同源。12个鸡Wnt基因的mRNA序列可在GenBank中获得，并且基于与来自其他物种的基因的同源性鉴定并克隆了另外6个Wnt基因（Wnt 2、Wnt 5 b、Wnt 7 b、Wnt 8b、Wnt 9 b和Wnt 16）的mRNA。此外，我们发现，鸡Wnt 3a和Wnt 7 b基因编码两个替代的mRNA异构体含有不同的第一外显子。原位杂交后，我们发现，在18个Wnt基因，11个基因在前眼表达，表现出不同的时空模式。几种Wnt在透镜中表达，包括前上皮中的Wnt 2和Wnt 2b以及分化中的透镜纤维细胞中的Wnt 5a、Wnt 5 b、Wnt 7a和Wnt 7 b。在角膜中，我们在眼表外胚层（包括角膜上皮）中检测到Wnt 3a、Wnt 6和Wnt 9 b，并在角膜内皮分化开始时检测到Wnt 9a。在视杯中，Wnt 2、Wnt 2b和Wnt 9a定位于视杯的边缘（推定虹膜），而Wnt 5a和Wnt 16在分化的视杯的睫状上皮/虹膜区中检测到，并且Wnt 6在虹膜间充质中表达。这些数据表明，Wnt信号可能在前眼发育中发挥重要作用。
To address the roles of Wnts in the development of the anterior eye, we used a chicken model to perform comprehensive expression analysis of all Wnt genes during anterior eye development. In analyzing the available genomic sequences, we found that the chicken genome encodes 18 Wnt proteins that are homologous to corresponding human and mouse proteins. The mRNA sequences for 12 chicken Wnt genes are available in GenBank, and mRNAs for six other Wnt genes (Wnt2, Wnt5b, Wnt7b, Wnt8b, Wnt9b, and Wnt16) were identified and cloned based on the homology to the genes from other species. In addition, we found that chicken Wnt3a and Wnt7b genes encode two alternative mRNA isoforms containing different first exons. Following in situ hybridization, we found that out of 18 Wnt genes, 11 genes were expressed in the anterior eye, exhibiting distinct temporal-spatial patterns. Several Wnts were expressed in the lens, including Wnt2 and Wnt2b in the anterior epithelium and Wnt5a, Wnt5b, Wnt7a, and Wnt7b in the differentiating lens fiber cells. In the cornea, we detected Wnt3a, Wnt6, and Wnt9b in the ocular surface ectoderm, including the corneal epithelium, and Wnt9a in the corneal endothelium from the onset of its differentiation. In the optic cup, Wnt2, Wnt2b, and Wnt9a were localized in the rim of the optic cup (presumptive iris), while Wnt5a and Wnt16 were detected in the ciliary epithelium/iris zone of the differentiated optic cup, and Wnt6 was expressed in the iridial mesenchyme. These data suggest that Wnt signaling might play important roles in anterior eye development.