Loss of Carotene-9′,10′-Monooxygenase Expression Increases Serum and Tissue Lycopene Concentrations in Lycopene-Fed Mice
Loss of Carotene-9′,10′-Monooxygenase Expression Increases Serum and Tissue Lycopene Concentrations in Lycopene-Fed Mice
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DOI：
10.3945/jn.110.128033
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发表时间：
2010-12-01
影响因子：
4.2
通讯作者：
Erdman, John W., Jr.
中科院分区：
文献类型：
--
作者：
Ford, Nikki A.;Clinton, Steven K.;Erdman, John W., Jr.
已发现两种酶用于哺乳动物类胡萝卜素的氧化代谢胡萝卜素-1515单加氧酶（CMO I）主要集中切割β β-胡萝卜素形成维生素A我们假设胡萝卜素-9 '10单加氧酶（CMO II）在代谢中发挥关键作用无环非维生素原A类胡萝卜素，例如番茄红素我们研究了年轻成年雄性野生型（WT）中类胡萝卜素的生物积累小鼠或缺乏CMO-II的小鼠（CM II KO）给小鼠喂食AIN 93 G饮食或补充有10%番茄粉130 mg番茄红素/kg饮食的相同饮食（10%番茄红素珠粒）或安慰剂珠粒持续4或30天番茄红素与WT小鼠组织相比优先在CMO-II KO小鼠组织和血清中积累。II KO小鼠组织与WT小鼠组织的比较CMO-I和CMO-II的相对组织mRNA表达在小鼠组织中差异表达，并且CMO-II而不是CMO-I在小鼠前列腺中表达。总之，CMO-II表达的丧失导致番茄喂养小鼠中番茄红素的血清和组织浓度增加J Nutr 140 2134-2138 2010
Two enzymes have been identified for the oxidative metabolism of carotenoids in mammals Carotene-15 15 monooxygenase (CMO I) primarily centrally cleaves beta beta-carotene to form vitamin A We hypothesize that carotene-9' 10 monooxygenase (CMO II) plays a key role in metabolism of acyclic nonprovitamin A carotenoids such as lycopene We investigated carotenoid bioaccumulation in young adult male wild-type (WT) mice or mice lacking CMO-II (CM II KO) Mice were fed an AIN 93G diet or identical diets supplemented with 10% tomato powder 130 mg lycopene/kg diet (10% lycopene beadlets) or placebo beadlets for 4 or 30 d Lycopene preferentially accumulated in CMO-II KO mouse tissues and serum compared with WT mouse tissues beta-Carotene preferentially accumulated in some CMO-II KO mouse tissues compared with WT mouse tissues Relative tissue mRNA expression of CMO-I and CMO-II was differentially expressed in mouse tissues and CMO-II but not CMO-I was expressed in mouse prostate In conclusion the loss of CMO-II expression leads to increased serum and tissue concentrations of lycopene in tomato-fed mice J Nutr 140 2134-2138 2010