Significant difference in mycorrhizal specificity between an autotrophic and its sister mycoheterotrophic plant species of Petrosaviaceae.
Significant difference in mycorrhizal specificity between an autotrophic and its sister mycoheterotrophic plant species of Petrosaviaceae.
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Petrosaviaceae 的自养植物及其姊妹菌异养植物物种之间的菌根特异性存在显着差异。
DOI：
10.1007/s10265-014-0661-z
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发表时间：
2014
影响因子：
2.8
通讯作者：
Yukawa T
中科院分区：
文献类型：
--
作者：
Yamato M;Ogura-Tsujita Y;Takahashi H;Yukawa T
油桐科（Petrosaviaceae）是一个单子叶植物科，包括两个属：自养的日本百合属（Japonolirion）和真菌异养的油桐属（Petrosavia）。因此，这个植物科提供了一个很好的系统，以检查特异性差异之间的自养和密切相关的真菌异养植物物种的菌生体。通过分子生物学鉴定，对单种属的唯一种--日本百合属（Japonolirionosense）的所有已知生境中的真菌进行了调查，共检测到22种丛枝菌根（arbuscularmycorrhizal，AM）真菌类群，分别分布于丛枝孢目（Acanthesporales）、多孢目（Diversisporales）和球囊霉目（Glomerales）。相比之下，在先前的研究中，在球囊霉目中仅检测到一种AM真菌的真菌类型，主要来自于真菌异养的Petrosavia sakuraiii。J.外群植物芒草（Miscanthus sinensis）中的Osenseand，表明在油桐科真菌营养型进化过程中真菌特异性增强。此外，还对J. Osense与优势真菌P. sakuraii，其中一个是嵌套在一个分支的P。樱生菌这些结果表明，真菌的合作伙伴不一定转移，而是选择在进化过程中的mycoheterotrophy。我们还证实了J. osense。
Petrosaviaceae is a monocotyledonous plant family that comprises two genera: the autotrophicJaponolirionand the mycoheterotrophicPetrosavia. Accordingly, this plant family provides an excellent system to examine specificity differences in mycobionts between autotrophic and closely related mycoheterotrophic plant species. We investigated mycobionts ofJaponolirion osense, the sole species of the monotypic genus, from all known habitats of this species by molecular identification and detected 22 arbuscular mycorrhizal (AM) fungal phylotypes in Archaesporales, Diversisporales, and Glomerales. In contrast, only one AM fungal phylotype in Glomerales was predominantly detected from the mycoheterotrophicPetrosavia sakuraiiin a previous study. The high mycobiont diversity inJ. osenseand in an outgroup plant,Miscanthus sinensis(Poaceae), indicates that fungal specificity increased during the evolution of mycohetrotrophy in Petrosaviaceae. Furthermore, some AM fungal sequences ofJ. osenseshowed >99 % sequence similarity to the dominant fungal phylotype ofP. sakuraii,and one of them was nested within a clade ofP. sakuraiimycobionts. These results indicate that fungal partners are not necessarily shifted, but rather selected for in the course of the evolution of mycoheterotrophy. We also confirmed theParis-type mycorrhiza inJ. osense.