Detection of Human Parechoviruses from Clinical Stool Samples in Aichi, Japan
Detection of Human Parechoviruses from Clinical Stool Samples in Aichi, Japan
复制标题
DOI：
10.1128/jcm.00086-10
复制
发表时间：
2010-08-01
影响因子：
9.4
通讯作者：
Minagawa, Hiroko
中科院分区：
文献类型：
--
作者：
Ito, Miyabi;Yamashita, Teruo;Minagawa, Hiroko
在1999年4月至2008年3月期间，从日本爱知县通过感染源监测收集的疑似病毒感染患者的粪便样本中，共检测了4976份粪便样本，以检测人类副病毒(HPev)的存在。我们用细胞培养、逆转录聚合酶链式反应(RT-PCR)或两者同时检测了110个样本中的HPeV。通过中和试验或核苷酸序列测定进行血清学分型，并对VP1区和5‘非翻译区进行系统发育分析，发现63株为HPeV 1型，其次是44株HPeV-3株、2株HPeV-4株和1株HPeV-6株。2006年日本HPeV-3分离株与此前报道的加拿大和荷兰HPeV-3毒株的核苷酸和氨基酸序列高度同源性，证实了HPeV-3感染在全球范围内的流行。97%的HPeV阳性患者年龄在3岁以下，86.2%的患者年龄在12个月以下。HPeV阳性患者的临床诊断为胃肠炎、呼吸系统疾病、发热性疾病、红疹、手足口病、无菌性脑膜炎和疱疹绞痛。49例HPeV阳性的胃肠炎患者中，HPeV-1阳性35例，HPeV-3阳性12例；呼吸系统疾病患者25例，HPeV-1阳性11例，HPeV-3阳性14例。HPeV-3可能是儿童呼吸道感染的重要病原体。HPeV-1主要在秋季和冬季检出，而HPeV-3型病例大部分在夏季和秋季检出。不同的临床表现和季节性提示HPeV-1和HPeV-3感染具有不同的致病机制。
Between April 1999 and March 2008, a total of 4,976 stool specimens collected from patients with suspected viral infection through infectious agent surveillance in Aichi, Japan, were tested for the presence of human parechoviruses (HPeVs). We detected HPeVs in 110 samples by either cell culture, reverse transcriptase PCR (RT-PCR), or both. Serotyping either by neutralization test or by nucleotide sequence determination and phylogenetic analysis of the VP1 region and 5' untranslated region (5'UTR) regions revealed that 63 were HPeV type 1 (HPeV-1), followed by 44 HPeV-3 strains, 2 HPeV-4 strains, and 1 HPeV-6 strain. The high nucleotide and amino acid sequence identities of the Japanese HPeV-3 isolates in 2006 to the strains previously reported from Canada and Netherlands confirmed the worldwide prevalence of HPeV-3 infection. Ninety-seven percent of the HPeV-positive patients were younger than 3 years, and 86.2% younger than 12 months. The clinical diagnoses of HPeV-positive patients were gastroenteritis, respiratory illness, febrile illness, exanthema, "hand, foot, and mouth disease," aseptic meningitis, and herpangina. Among 49 HPeV-positive patients with gastroenteritis, 35 were positive with HPeV-1 and 12 with HPeV-3, and out of 25 with respiratory illness, 11 were positive with HPeV-1 and 14 with HPeV-3. HPeV-3 seemed to be an important etiological agent of respiratory infection of children. While HPeV-1 was detected predominantly during fall and winter, the majority of the HPeV-3 cases were detected during summer and fall. A different pattern of clinical manifestations as well as seasonality suggested that there are different mechanisms of pathogenesis between HPeV-1 and HPeV-3 infections.