Marinobufagenin (MBG) suppression of ethanol-seeking behavior is associated with inhibition of brain cortex Na/K-ATPase in mice.
Marinobufagenin (MBG) suppression of ethanol-seeking behavior is associated with inhibition of brain cortex Na/K-ATPase in mice.
复制标题
海蟾蜍配基 (MBG) 对乙醇寻求行为的抑制与小鼠大脑皮层 Na/K-ATP 酶的抑制有关。
DOI：
10.1016/s0924-977x(02)00026-3
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Zvartau,EdwinE
中科院分区：
文献类型：
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作者：
Kashkin,VladimirA;Bagrov,AlexeiY;Fedorova,OlgaV;Bagrov,YakovY;Agalakova,NataliaI;Patkina,NadezhdaA;Zvartau,EdwinE
在本研究中的假设进行了测试，钠泵配体（SPL）可以调节酒精寻求行为，这种效果是在中枢神经系统中的Na/K-ATP酶活性的变化有关。在用媒介物或内源性SPL、海蟾毒配基（MBG）腹膜内预处理后，测试小鼠乙醇静脉内自我给药（IVSA）的起始。药物-和实验-幼稚的小鼠获得IVSA的2%乙醇在一个单一的30分钟的会议。发现MBG剂量依赖性地减弱（1.25-2.5 μg/kg）乙醇IVSA的启动，导致反应依赖性动物和轭动物的操作性反应之间的比率和差异降低，以及表现出乙醇寻求行为的小鼠百分比降低。由MBG产生的乙醇的增强作用的衰减与能够同时抑制大脑皮层中的Na/K-ATP酶的这种类固醇的脑水平相关。我们推测内源性洋地黄样因子可以调节乙醇的增强作用。
In the present study the hypothesis was tested that sodium pump ligands (SPL) can modulate alcohol-seeking behavior and that this effect is related to changes in Na/K-ATPase activity in the central nervous system. Mice were tested for initiation of ethanol intravenous self-administration (IVSA) following i.p. pretreatment with vehicle or the endogenous SPL, marinobufagenin (MBG). Drug- and experimentally-naive mice acquired IVSA of 2% ethanol during a single 30-min session. MBG was found to dose-dependently attenuate (1.25–2.5 μg/kg) initiation of ethanol IVSA producing a decrease in the ratio and in the difference between operant responses of response-dependent and yoked animals as well as a decrease in percentage of mice demonstrating ethanol-seeking behavior. Attenuation of the reinforcing effect of ethanol resulting from MBG was associated with brain levels of this steroid capable of concurrently inhibiting Na/K-ATPase in the brain cortex. We hypothesize that endogenous digitalis-like factors could modulate the reinforcing effect of ethanol.