Collagen Synthesis: Localization of Prolyl Hydroxylase in Tendon Cells Detected with Ferritin-Labeled Antibodies
Collagen Synthesis: Localization of Prolyl Hydroxylase in Tendon Cells Detected with Ferritin-Labeled Antibodies
复制标题
DOI：
10.1126/science.182.4114.825
复制
发表时间：
1973-11
期刊：
影响因子：
56.9
通讯作者：
Bj�rn R. Olsen;R. Berg;Y. Kishida;D. Prockop
中科院分区：
文献类型：
--
作者：
Bj�rn R. Olsen;R. Berg;Y. Kishida;D. Prockop
一种改进的方法被用来将铁蛋白连接到抗脯氨酸羟基酶的抗体上，脯氨酸羟基酶是一种在胶原中合成羟基脯氨酸的酶。在电子显微镜下，该酶定位于胚胎肌腱细胞粗面内质网池中，这表明当新合成的多肽进入脑池时，脯氨酸发生了羟化。
An improved procedure was employed for linking ferritin to antibodies against prolyl hydroxylase, the enzyme that synthesizes the hydroxyproline in collagen. By electron microscopy, the enzyme was then found to be localized in cisternae of the rough endoplasmic reticulum of embryonic tendon cells; this indicates that hydroxylation of proline occurs while newly synthesized polypeptides are fed into the cisternae.