Acute ethanol induces Fos in GABAergic and non-GABAergic forebrain neurons:: A double-labeling study in the medial prefrontal cortex and extended amygdala
Acute ethanol induces Fos in GABAergic and non-GABAergic forebrain neurons:: A double-labeling study in the medial prefrontal cortex and extended amygdala
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DOI：
10.1016/j.neuroscience.2008.01.069
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发表时间：
2008-04-22
期刊：
影响因子：
3.3
通讯作者：
Berod, A.
中科院分区：
文献类型：
--
作者：
Leriche, M.;Mendez, M.;Berod, A.
本研究的目的是进一步解决这样的假设：乙醇会激活特定大脑神经回路中的 GABA 能神经元，这些神经回路介导动机行为和行动控制，例如中央扩展杏仁核和内侧前额叶皮层。雄性Sprague-Dawley大鼠接受7天的习惯性每日灌胃水(5ml/kg)，随后单次急性灌胃剂量乙醇(2.5g/kg)或水，然后2小时后，通过多聚甲醛灌注。在整个实验过程中，在动物室中不受干扰的大鼠也进行了灌注（初始组）。对脑切片进行单 Fos 免疫组织化学或双 Fos 免疫组织化学/谷氨酸脱羧酶 (GAD) mRNA 原位杂交。与初始组相比，灌胃水增加了边缘下皮层和杏仁核中央核外侧部分的 Fos 免疫反应细胞数量。与水处理组相比，乙醇给药增加了边缘下皮质（+57.5%）和边缘前皮质（+105.3%）、伏隔核壳区（+88.2%）、杏仁核中央核内侧部分（+160%）和终纹床核外侧部分（+198.8%）的 Fos 免疫反应细胞数量。在伏隔核壳区、杏仁核中央核和终纹床核中，乙醇给药后超过 80% 的 Fos 免疫反应性神经元呈 GABA 能。相比之下，在前边缘皮质中，75% 的 Fos 免疫反应性神经元不是 GABA 能神经元。这些结果构成了乙醇和 GABA 神经元之间区域特异性功能相互作用的新证据。 (C) 2008 国际广播组织。由爱思唯尔有限公司出版。保留所有权利。
The purpose of this study was to further address the hypothesis that ethanol activates GABAergic neurons in specific brain neurocircuits that mediate motivated behavior and control of action, such as the central extended amygdala and medial prefrontal cortex. Male Sprague-Dawley rats received habituation to 7 days of daily intragastric administration of water (5 ml/kg) followed by a single acute intragastric dose of ethanol (2.5 g/kg) or water then, 2 h later, by paraformaldehyde perfusion. Rats left undisturbed in the animal room throughout the experiment were also perfused (naive group). Brain sections were processed for single Fos immunohistochemistry or dual Fos immunohistochemistry/glutamic acid decarboxylase (GAD) mRNA in situ hybridization. Intragastric water administration increased the number of Fos-immunoreactive cells in the infralimbic cortex and lateral part of the central nucleus of the amygdala compared with the naive group. Ethanol administration increased the number of Fos-immunoreactive cells in the infralimbic (+57.5%) and prelimbic (+105.3%) cortices, nucleus accumbens shell region (+88.2%), medial part of the central nucleus of the amygdala (+160%), and lateral part of the bed nucleus of the stria terminalls (+198.8%) compared with the water-treated group. In the nucleus accumbens shell region, central nucleus of the amygdala, and bed nucleus of the stria terminalis, more than 80% of Fos-immunoreactive neurons were GABAergic after ethanol administration. In contrast, in the prelimbic cortex, 75% of Fos-immunoreactive neurons were not GABAergic. These results constitute new evidence for region-specific functional interactions between ethanol and GABAergic neurons. (C) 2008 IBRO. Published by Elsevier Ltd. All rights reserved.