Differential sensitivity of metaphase to diamide and ouabain in HeLa cells.
Differential sensitivity of metaphase to diamide and ouabain in HeLa cells.
复制标题
HeLa 细胞中期对二酰胺和哇巴因的敏感性差异。
DOI：
10.1016/0014-4827(85)90449-5
复制
发表时间：
1985
影响因子：
3.7
通讯作者：
Sisken,JE
中科院分区：
文献类型：
--
作者：
Ziegler,ML;Sisken,JE
We have examined the effects of diamide, an oxidizer of glutathione, on the progress of HeLa cells through the cell cycle. At concentrations which do not significantly alter generation time, anaphase or cytokinesis, diamide causes a two-fold increase in the duration of metaphase. At 3× 10− 8 M, ouabain also prolongs metaphase without effect on anaphase or cytokinesis, though with a different time course. The data suggest that the metaphase stage of mitosis is particularly sensitive to alterations in both sulfhydryl groups and Na+ levels but that the effects of diamide are probably not primarily due to the oxidation of sulfhydryl groups of the Na+ K+-ATPase.
