Proteomic analysis of root meristems and the effects of acetohydroxyacid synthase-inhibiting herbicides in the root of Medicago truncatula

Proteomic analysis of root meristems and the effects of acetohydroxyacid synthase-inhibiting herbicides in the root of Medicago truncatula
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DOI:
10.1021/pr0600677
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发表时间:
2006-09-01
影响因子:
4.4
通讯作者:
Rolfe, Barry G.
Rolfe, Barry G.
中科院分区:
生物学2区
文献类型:
--
作者:
Holmes, Peta;Farquharson, Ryan;Rolfe, Barry G.

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对蒺藜苜蓿(Medicago truncatula)主根和侧根的分生组织和非分生组织以及乙酰羟酸脱氢酶抑制剂处理的植物的分生组织进行了定量蛋白质组分析。81个蛋白点在分生组织和非分生组织中的积累发生了变化,51个蛋白点在除草剂处理的分生组织中的积累发生了显著变化。鉴定的蛋白质表明两个趋势,(i)增加积累的细胞分裂和氧化还原介导的蛋白质在分生组织相比,nonmeristematic组织和(ii)增加积累的发病相关的代谢蛋白质和除草剂处理的根减少积累。
Quantitative proteome analyses of meristematic and nonmeristematic tissues from Medicago truncatula primary and lateral roots and meristem tissues from plants treated with acetohydroxyacid synthaseinhibiting herbicides were made. The accumulation of 81 protein spots changed in meristematic and nonmeristematic tissues and 51 protein spots showed significant changes in accumulation in herbicide-treated meristems. Identified proteins indicate two trends, (i) increased accumulation of cell division and redox-mediating proteins in meristems compared to nonmeristematic tissues and (ii) increased accumulation of pathogenesis-related and decreased accumulation of metabolic proteins in herbicide-treated roots.