Use of Cocultures to Measure the Blood-Brain Barrier Permeability of Oxytocin.
Use of Cocultures to Measure the Blood-Brain Barrier Permeability of Oxytocin.
复制标题
使用共培养物测量催产素的血脑屏障渗透性。
DOI：
10.1007/978-1-0716-1759-5_15
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Higashida H.
中科院分区：
文献类型：
--
作者：
Yamamoto Y;Harashima A;Munesue SI;Higashida H.
原代猴脑毛细血管内皮细胞培养物，与大鼠周细胞和星形胶质细胞，提供了一种测定系统，用于预测催产素（OT）的能力，通过血脑屏障（BBB），使用市售的体外BBB试剂盒。具有高跨内皮电阻（TEER）的体外“BBB”的完整性可在实验准备后约4天建立。OT的主要内皮转运是从上腔（腔血侧）到下腔（腔外脑侧），呈剂量依赖性。OT通过内皮细胞中的晚期糖基化终产物受体（receptor for advanced glycation end-products，ERK）进行转运，这一点通过使用带有短发夹RNA（short hairpin RNA，shRNA）处理的ERK敲低系统得到证实。该体外测定系统可用于进一步评估OT穿过BBB的转运。
Primary monkey brain capillary endothelial cell cultures, with rat pericytes and astrocytes, provide an assay system for predicting the ability of oxytocin (OT) to cross the blood–brain barrier (BBB), using a commercially available in vitro BBB kit. The integrity of the in vitro “BBB,” which has a high transendothelial electrical resistance (TEER), can be established approximately 4 days after preparations for experiments. Dominant endothelial transport of OT is from the upper (luminal blood side) to lower (abluminal brain side) chambers, dose-dependently. OT is transported by the receptor for advanced glycation end-products (RAGE) in endothelial cells, which is evidenced using the RAGE knockdown system with short hairpin RNA (shRNA) treatment. This in vitro assay system is useful for further assessment of OT transport across the BBB.