Characterization of human leukocyte antigen-G (HLA-G) expression in endometrial adenocarcinoma
Characterization of human leukocyte antigen-G (HLA-G) expression in endometrial adenocarcinoma
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DOI：
10.1016/j.ygyno.2006.01.045
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发表时间：
2006-10-01
影响因子：
4.7
通讯作者：
Kost, Edward R.
中科院分区：
文献类型：
--
作者：
Barrier, Breton F.;Kendall, Brian S.;Kost, Edward R.
目标。目前的研究试图确定子宫内膜腺癌是否表达人类白细胞抗原-G(人类白细胞抗原-G)，一种免疫调节蛋白，以及表达程度是否与肿瘤的分期有关。应用41484抗HLAG单抗对44例子宫内膜腺癌进行免疫组织化学染色。转移性植入物不包括在内。用RNA原位杂交检测了10个样本的子集，以确认是否存在HLA-G转录本。根据分级、肿瘤组织学和疾病分期对染色结果进行分析。采用Spearman等级相关分析来评估肿瘤分级、组织学和疾病分期与人类白细胞抗原G蛋白染色的关系。通过受试者-操作者特征(ROC)曲线分析，确定人类白细胞抗原-G蛋白染色作为晚期疾病临床标志物的可行性。免疫组织化学显示，55%(24/44)的子宫内膜腺癌组织中有人类白细胞抗原-G蛋白表达，且仅定位于腺样上皮，而不是间质上皮。RNA原位杂交证实在大多数被测试的样本中存在转录本，并且也定位于腺上皮。人类白细胞抗原-G蛋白阳性率与子宫内膜癌分期呈显著正相关，P<；0.01。研究发现，在判断转移和非转移疾病方面，HL A-G是一种较好的判别器，其ROC曲线下面积为0.75。人类白细胞抗原-G蛋白在子宫内膜腺癌中大量表达，定位于腺上皮细胞。HL A-G可作为子宫内膜癌转移性癌术前预测的临床指标。(C)2006 Elsevier Inc.保留所有权利。
Objectives. The current study sought to determine if endometrial adenocarcinomas express human leukocyte antigen-G (HLA-G), an immune-regulatory protein, and if degree of expression correlates with the stage of carcinoma.Methods. Forty-four primary endometrial adenocarcinomas were tested using immunohistochemical staining with the 41484 anti-HLA-G monoclonal antibody. Metastatic implants were not included. A subset of 10 samples was tested using RNA in situ hybridization to confirm the presence of HLA-G transcript. Results of staining were analyzed with respect to grade, tumor histology, and stage of disease. Spearman rank correlation was used to assess tumor grade, histology, and disease stage as a function of HLA-G protein staining. Receiver-operator characteristic (ROC) curve analysis was used to determine the feasibility of HLA-G protein staining as a clinical marker for advanced stage disease.Results. Immunohistochemical staining for HLA-G protein was seen in 55% (24/44) of primary site endometrial adenocarcinomas and localized to glandular but not stromal epithelium. RNA in situ hybridization confirmed the presence of transcript in the majority of samples tested and also localized to glandular epithelium. A significant correlation was seen with increasing HLA-G protein staining and increasing stage of endometrial cancer, P < 0.01. HLA-G was found to be a fair discriminator as a test for metastatic disease with an area under the ROC curve of 0.75 for metastatic versus non-metastatic disease.Conclusions. HLA-G protein is expressed in a significant number of endometrial adenocarcinomas, in which it is localized to the glandular epithelium. HLA-G may serve as a clinical marker for the preoperative prediction of metastatic endometrial cancer. (c) 2006 Elsevier Inc. All rights reserved.