PURIFICATION AND CHARACTERIZATION OF THE LETHAL TOXIN (ALPHA-TOXIN) OF CLOSTRIDIUM-SEPTICUM
PURIFICATION AND CHARACTERIZATION OF THE LETHAL TOXIN (ALPHA-TOXIN) OF CLOSTRIDIUM-SEPTICUM
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DOI：
10.1128/iai.60.3.784-790.1992
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发表时间：
1992-03-01
影响因子：
3.1
通讯作者：
TWETEN, RK
中科院分区：
文献类型：
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作者：
BALLARD, J;BRYANT, A;TWETEN, RK
毒梭菌致死（α毒素）被纯化，发现是一种约48 kDa的碱性蛋白（pI 8.4），既致命又溶血。α毒素的溶血活性约为每毫克2 × 10(7)溶血单位，对小鼠的50%致死剂量约为每公斤体重10 μ g。毒素形成浓度依赖性，抗十二烷基硫酸钠聚集体约230 kDa。与模拟免疫小鼠相比，α毒素免疫小鼠在感染败血症时的存活时间显著增加。用兔多克隆抗体对纯化的毒素进行了免疫，证实7株不同的败血菌分离株中存在相同分子量的毒素。产气荚膜梭状芽胞杆菌、溶组织梭状芽胞杆菌、chauvoei梭状芽胞杆菌或艰难梭状芽胞杆菌培养的上清液中未发现与败血症梭状芽胞杆菌α毒素特异性抗体发生反应的蛋白。
Clostridium septicum lethal (alpha-toxin) was purified and found to be a basic protein (pI 8.4) of approximately 48 kDa that is both lethal and hemolytic. The alpha-toxin had a hemolytic activity of approximately 2 x 10(7) hemolytic units per mg and a 50% lethal dose of approximately 10-mu-g/kg of body weight for mice. The alpha-toxin formed concentration-dependent, sodium dodecyl sulfate-resistant aggregates of approximately 230 kDa. Mice immunized with alpha-toxin showed a significant increase in survival time over mock-immunized mice when challenged with C. septicum. Rabbit polyclonal antibody was generated against the purified toxin and was used to confirm that toxin with the same molecular weight was present in seven different C. septicum isolates. No proteins in the supernatants from cultures of Clostridium perfringens, Clostridium histolyticum, Clostridium chauvoei, or Clostridium difficile were found to react with the C. septicum alpha-toxin-specific antibody.