DNA microarray analysis reveals novel gene expression profiles in collagen-induced arthritis
DNA microarray analysis reveals novel gene expression profiles in collagen-induced arthritis
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DOI：
10.1006/clim.2002.5227
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发表时间：
2002-11-01
影响因子：
8.6
通讯作者：
Hirsch, R
中科院分区：
文献类型：
--
作者：
Thornton, S;Sowders, D;Hirsch, R
分析了早期和晚期胶原诱导性关节炎（CIA）的整体基因表达。在分析的8734个cDNA中，330个被诱导，55个在早期或晚期疾病中下调超过两倍。这385个cDNAs的层次聚类显示了五种不同的表达模式，区分早期和晚期疾病，并与爪的组织病理学变化相关。在385个cDNA中，185个是已知的特征基因，其中大多数没有被描述为在关节炎中发挥作用。然而，这些基因中的几个涉及与关节炎相关的病理过程，包括细胞凋亡、炎症和细胞增殖。一个有趣的基因，卵泡抑素样基因，在炎症性滑膜血管翳和侵蚀性骨之间的接触边缘沿着高度表达，表明在关节破坏中的作用。这些结果表明，全球基因表达谱区分早期和晚期CIA，并揭示了几个新的基因关节炎的进一步表征，这将促进我们对关节炎的理解。(C)2002 Elsevier Science（美国）。
Global gene expression was analyzed in early and late collagen-induced arthritis (CIA). Of 8734 cDNAs analyzed, 330 were induced and 55 downregulated greater than twofold in early or late disease. Hierarchical clustering of these 385 cDNAs demonstrated five distinct expression patterns differentiating early from late disease and correlating with histopathologic changes in the paw. Of the 385 cDNAs, 185 are known, characterized genes, the majority of which are not described as playing a role in arthritis. However, several of these genes are involved in pathological processes relating to arthritis, including apoptosis, inflammation, and cellular proliferation. One interesting gene, follistatin-like gene, is highly expressed along the margin of contact between inflammatory synovial pannus and eroding bone, suggesting a role in joint destruction. These results demonstrate that global gene expression profiles distinguish early and late CIA and reveal several genes novel to arthritis the further characterization of which will advance our understanding of arthritis. (C) 2002 Elsevier Science (USA).