Growth factor release and enhanced encapsulated periodontal stem cells viability by freeze-dried platelet concentrate loaded thermo-sensitive hydrogel for periodontal regeneration.
Growth factor release and enhanced encapsulated periodontal stem cells viability by freeze-dried platelet concentrate loaded thermo-sensitive hydrogel for periodontal regeneration.
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DOI：
10.1016/j.sdentj.2018.06.002
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发表时间：
2018-10
期刊：
影响因子：
--
通讯作者：
Moussa TA
中科院分区：
文献类型：
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作者：
Ammar MM;Waly GH;Saniour SH;Moussa TA
牙周再生是一个极具挑战性的过程，因为它需要同时再生三种不同的组织。本研究的目的是开发一种复合材料，可以很容易地应用，并能充分提供必要的生长因子和牙周组织再生祖细胞。制备冻干血小板浓缩物（FDPC），并以5、10或15 mg/ml的浓度掺入热敏性壳聚糖/β-甘油磷酸盐（β-GP）水凝胶中。随着温度从25 °C升高至37 °C，研究水凝胶的粘度，并在四个时间点（1小时、1天、1周、2周）研究转化生长因子（TGF-β1）、血小板衍生生长因子（PDGF-BB）和胰岛素样生长因子（IGF-1）的释放动力学。从人第三磨牙中分离牙周膜干细胞（PDLSC），并将其封装在不同的水凝胶组中。与标准培养条件和非FDPC负载的水凝胶相比，在培养7天后研究它们的活力。结果表明，加载FDPC在水凝胶中降低初始粘度相比，未加载的对照组，并没有影响任何组的溶胶-凝胶转变。所有负载FDPC的水凝胶组均表现出持续释放TGF-β1和PDGF-BB两周，不同浓度之间存在显著差异。与未负载的水凝胶相比，水凝胶中10和15 mg/ml FDPC的负载显著增加了PDLSC的活力，并且与标准培养条件相当。因此，可以得出结论，在壳聚糖/β-GP水凝胶中负载FDPC可以提供增强的可注射性、生长因子的持续释放和包封的干细胞的增加的活力，这可以有益于牙周组织再生。
Periodontium regeneration is a highly challenging process as it requires the regeneration of three different tissues simultaneously. The aim of this study was to develop a composite material that can be easily applied and can sufficiently deliver essential growth factors and progenitor cells for periodontal tissue regeneration. Freeze-dried platelet concentrate (FDPC) was prepared and incorporated in a thermo-sensitive chitosan/β-glycerol phosphate (β-GP) hydrogel at concentrations of 5, 10, or 15 mg/ml. The viscosity of the hydrogels was investigated as the temperature rises from 25 °C to 37 °C and the release kinetics of transforming growth factor (TGF-β1), platelet-derived growth factor (PDGF-BB) and insulin-like growth factor (IGF-1) were investigated at four time points (1 h, 1 day, 1 week, 2 weeks). Periodontal ligament stem cells (PDLSCs) were isolated from human third molars and encapsulated in the different hydrogel groups. Their viability was investigated after 7 days in culture in comparison to standard culture conditions and non FDPC-loaded hydrogel. Results showed that loading FDPC in the hydrogel lowered the initial viscosity in comparison to the unloaded control group and did not affect the sol-gel transition in any group. All FDPC-loaded hydrogel groups exhibited sustained release of TGF-β1 and PDGF-BB for two weeks with significant difference between the different concentrations. The loading of 10 and 15 mg/ml of FDPC in the hydrogel increased the PDLSCs viability significantly compared to the unloaded hydrogel and was comparable to the standard culture conditions. Accordingly, it may be concluded that loading FDPC in a chitosan/β-GP hydrogel can offer enhanced injectability, a sustained release of growth factors and increased viability of encapsulated stem cells which can be beneficial in periodontium tissue regeneration.