Molecular characterization of a trisegmented chrysovirus isolated from the radish Raphanus sativus
Molecular characterization of a trisegmented chrysovirus isolated from the radish Raphanus sativus
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从萝卜 Raphanus sativus 中分离的三节金病毒的分子特征
DOI：
10.1016/j.virusres.2013.06.004
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发表时间：
2013-09-01
期刊：
影响因子：
5
通讯作者：
Zhu, Xiwu
中科院分区：
文献类型：
--
作者：
Li, Liqiang;Liu, Jianning;Zhu, Xiwu
萝卜（Raphanus sativus L）在世界各地都有种植，具有重要的农艺意义。与部分病毒相关的dsRNAs先前在许多sativus品种中被发现。本研究利用改良的单引物扩增技术克隆了萝卜中的3个大片段dsRNAs。这三个长度分别为3638、3517和3299 bp的dsRNAs共享保守的未翻译末端区，每个dsRNAs都包含一个主要的开放阅读框，据推测分别编码chrsovirus复制酶、衣壳蛋白和蛋白酶。从感染的萝卜中分离到直径约45 nm的等长病毒样颗粒（VIP）。Northern blotting表明这些dsrna被封装在VLP中。含有这些dsRNA基因组片段的病毒被命名为Raphanus sativus chrysovirus 1 （RasCV1）。系统发育分析表明，RasCV1是黄病毒科的一个新种，与另一种推定的植物黄病毒红掌花叶相关病毒（AmaCV）形成一个植物分类群。台盼蓝染色的萝卜叶片组织未见真菌菌丝体。这些结果表明，RasCV1更可能是一种植物黄体病毒，而不是共生真菌中的黄体病毒。对RasCV1和AmaCV的BLAST分析表明，黄病毒样病毒可能广泛存在于菊科植物和单子叶植物中，并且黄病毒片段内内化到植物基因组中可能曾经发生过。(C) 2013 Elsevier B.V.版权所有
Radish (Raphanus sativus L) is cultivated worldwide and is of agronomic importance. dsRNAs associated with partitiviruses were previously found in many R. sativus varieties. In this study, three large dsRNAs from radish were cloned using a modified single primer amplification technique. These three dsRNAs of lengths 3638, 3517 and 3299 bp shared conserved untranslated terminal regions, and each contained a major open reading frame putatively encoding the chrysoviral replicase, capsid protein and protease respectively. Isometric virus-like particles (VIP), approximately 45 nm in diameter, were isolated from the infected radish plants. Northern blotting indicated that these dsRNAs were encapsidated in the VLP. The virus containing these dsRNA genome segments was named Raphanus sativus chrysovirus 1 (RasCV1). Phylogenetic analysis revealed that RasCV1 is a new species of the Chrysoviridae family and forms a plant taxon with another putative plant chrysovirus, Anthurium mosaic-associated virus (AmaCV). Furthermore, no fungal mycelia were observed in radish leaf tissues stained with trypan blue. These results indicated that RasCV1 is most likely a plant chrysovirus rather than a chrysovirus in symbiotic fungi. An exhaustive BLAST analysis of RasCV1 and AmaCV revealed that chrysovirus-like viruses might widely exist in eudicot and monocot plants and that endogenization of chrysovirus segments into plant genome might have ever happened. (C) 2013 Elsevier B.V. All rights reserved.