Microsatellite loci for the white-dotted mosquito (Culex restuans), a principal vector of West Nile virus in North America.
Microsatellite loci for the white-dotted mosquito (Culex restuans), a principal vector of West Nile virus in North America.
复制标题
白点蚊子（Resuans 库蚊）的微卫星位点，白点蚊子是北美西尼罗河病毒的主要媒介。
DOI：
10.1111/j.1755-0998.2008.02512.x
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发表时间：
2009
影响因子：
7.7
通讯作者：
Kramer,LauraD
中科院分区：
文献类型：
--
作者：
Fonseca,DinaM;Okada,Kenli;Kramer,LauraD
我们在白色斑点蚊子，库蚊restuans，一个关键的早春西尼罗河病毒载体的第一个微卫星位点的特点。富集方案产生了960个阳性克隆，我们对其中300个进行了测序。我们设计了引物来扩增29个独特的二、三和四核苷酸微卫星，并选择了17个在种群间扩增一致且多态的微卫星。我们为所有17个位点开发了三种多重引物组合。对44个个体的调查显示，在基因座上有2到20个等位基因，预期杂合度范围为0.17到0.89。这些标记将允许检查这种神秘的早期季节性脑炎媒介的生活史。
We characterized the first microsatellite loci in the white‐dotted mosquito,Culex restuans, a critical early spring West Nile virus vector. An enrichment protocol yielded 960 positive clones of which we sequenced 300. We designed primers to amplify 29 unique di‐, tri‐ and tetranucleotide microsatellites and chose 17 that amplified consistently across populations and were polymorphic. We developed three multiplex primer combinations for all 17 loci. A survey of 44 individuals revealed two to 20 alleles across loci, and expected heterozygosity ranging from 0.17 to 0.89. These markers will allow examination of the life history of this mysterious early season encephalitis vector.