Aging influences the response of T cells to stimulation by the ellagitannin, oenothein B.
Aging influences the response of T cells to stimulation by the ellagitannin, oenothein B.
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DOI：
10.1016/j.intimp.2015.04.008
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发表时间：
2015-06
影响因子：
5.6
通讯作者：
Jutila MA
中科院分区：
文献类型：
--
作者：
Ramstead AG;Schepetkin IA;Todd K;Loeffelholz J;Berardinelli JG;Quinn MT;Jutila MA
几种植物提取物，包括某些多酚，可以启动先天淋巴细胞并增强对次级刺激的反应。月见草素 B 是一种从柳兰和其他植物来源中分离出来的多酚，可单独增强牛和人类 NK 细胞和 T 细胞的 IFNγ 产生，以及响应细胞因子或肿瘤细胞的二次刺激。已知先天免疫细胞的反应性会受到衰老的影响，但这些细胞的多酚反应是否也受到衰老的影响尚不清楚。因此，我们检查了来自脐带血、年轻和成年捐献者的 T 细胞中月见草素 B 的反应性。我们发现，月见草苷 B 可以刺激不同年龄段个体的牛和人类 T 细胞，通过增加 IL-2Rα 和 CD69 表达来衡量。然而，T 细胞诱导细胞因子产生的明显差异。在人脐带血和小牛的 T 细胞中，月见草素 B 无法诱导 IFNγ 产生。然而，月见草苷 B 诱导成年人和牛的 T 细胞产生 IFNγ。此外，月见草苷 B 诱导人类成体 T 细胞产生 GM-CSF，但不诱导脐带血 T 细胞产生 GM-CSF。在反应性 T 细胞群中，我们发现 CD45RO+ 记忆 T 细胞比 CD45RO− T 细胞表达更多的细胞因子来响应月见草苷 B。总之，我们的数据表明，月见草碱 B 对 T 细胞的免疫刺激受到年龄的影响，特别是在免疫细胞因子的产生方面。
Several plant extracts, including certain polyphenols, prime innate lymphocytes and enhance responses to secondary stimuli. Oenothein B, a polyphenol isolated from Epilobium angustifolium and other plant sources, enhances IFNγ production by both bovine and human NK cells and T cells, alone and in response to secondary stimulation by cytokines or tumor cells. Innate immune cell responsiveness is known to be affected by aging, but whether polyphenol responses by these cells are also impacted by aging is not known. Therefore, we examined oenothein B responsiveness in T cells from cord blood, young, and adult donors. We found that oenothein B stimulates bovine and human T cells from individuals over a broad range of ages, as measured by increased IL-2Rα and CD69 expression. However, clear differences in induction of cytokine production by T cells were seen. In T cells from human cord blood and bovine calves, oenothein B was unable to induce IFNγ production. However, oenothein B induced IFNγ production by T cells from adult humans and cattle. In addition, oenothein B induced GM-CSF production by human adult T cells, but not cord blood T cells. Within the responsive T cell population, we found that CD45RO+ memory T cells expressed more cytokines in response to oenothein B than CD45RO− T cells. In summary, our data suggest that the immunostimulation of T cells by oenothein B is influenced by age, particularly with respect to immune cytokine production.