Identification of novel sRNAs involved in biofilm formation, motility, and fimbriae formation in Escherichia coli.
Identification of novel sRNAs involved in biofilm formation, motility, and fimbriae formation in Escherichia coli.
复制标题
DOI：
10.1038/srep15287
复制
发表时间：
2015-10-15
影响因子：
4.6
通讯作者：
Lee Y
中科院分区：
文献类型：
--
作者：
Bak G;Lee J;Suk S;Kim D;Young Lee J;Kim KS;Choi BS;Lee Y
细菌小 RNA (sRNA) 是许多生理过程中已知的调节因子。在大肠杆菌中，已经预测出大量的 sRNA，但其中只有大约一百种经过实验验证。尽管进行了大量研究，但它们的大部分功能仍未被发现。因此，对 sRNA 在特定细胞过程中的作用进行集体分析可能会提供一种有效的方法来识别其功能。在这里，我们构建了过表达 99 个 sRNA 的质粒集合，并分析了它们对生物膜形成和相关表型的影响。鉴定出 33 种显着影响这些细胞过程的 sRNA。除了抑制 I 型菌毛的所有 5 种 sRNA 都抑制生物膜形成外，没有观察到一致的相关性。有趣的是，尚未表征的 IS118 抑制了所有过程。我们的数据不仅揭示了单个 sRNA 在生物膜形成和其他表型中的潜在关键功能，而且还强调了导致这些过程的 sRNA 介导的代谢途径的意外复杂性。
Bacterial small RNAs (sRNAs) are known regulators in many physiological processes. In Escherichia coli, a large number of sRNAs have been predicted, among which only about a hundred are experimentally validated. Despite considerable research, the majority of their functions remain uncovered. Therefore, collective analysis of the roles of sRNAs in specific cellular processes may provide an effective approach to identify their functions. Here, we constructed a collection of plasmids overexpressing 99 individual sRNAs, and analyzed their effects on biofilm formation and related phenotypes. Thirty-three sRNAs significantly affecting these cellular processes were identified. No consistent correlations were observed, except that all five sRNAs suppressing type I fimbriae inhibited biofilm formation. Interestingly, IS118, yet to be characterized, suppressed all the processes. Our data not only reveal potentially critical functions of individual sRNAs in biofilm formation and other phenotypes but also highlight the unexpected complexity of sRNA-mediated metabolic pathways leading to these processes.