Soil microbial community responses to antibiotic-contaminated manure under different soil moisture regimes
Soil microbial community responses to antibiotic-contaminated manure under different soil moisture regimes
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DOI：
10.1007/s00253-014-5717-4
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发表时间：
2014-04
影响因子：
5
通讯作者：
Rüdiger Reichel;V. Radl;Ingrid Rosendahl;A. Albert;W. Amelung;M. Schloter;S. Thiele-Bruhn
中科院分区：
文献类型：
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作者：
Rüdiger Reichel;V. Radl;Ingrid Rosendahl;A. Albert;W. Amelung;M. Schloter;S. Thiele-Bruhn
磺胺嘧啶（SDZ）是一种经常用于牲畜的抗生素，当它与动物粪便一起进入土壤时会改变微生物群落，但研究人员无法理解这些影响与现行气候制度的相互作用。强烈控制微生物活动的气候因素是土壤湿度。在这里，我们假设SDZ对土壤微生物群落的影响将根据土壤水分条件进行调制。为了验证这一假设，我们进行了49天的完全控制气候室盆栽试验，土壤种植鸭茅（L.）。将不含或含有SDZ污染的粪肥改良的盆在动态水分制度（DMR）下孵育，与平均土壤水分为38%WHCmax的对照水分制度（CMR）相比，具有> 20%最大持水量（WHCmax）的重复干燥和再润湿变化。然后，我们监测SDZ浓度的变化，以及在表型磷脂脂肪酸和基因型16S rRNA基因片段模式的微生物群落后7，20，27，34和49天的孵育。结果表明，强烈的供水变化使SDZ在短期内可以进行温和的提取。因此，尽管SDZ对群落结构的影响很小，但PLFA衍生的微生物生物量在SDZ污染的DMR土壤中相对于CMR土壤受到抑制，表明动态水分变化加速了土壤微生物群落对抗生素的敏感性。
Sulfadiazine (SDZ) is an antibiotic frequently administered to livestock, and it alters microbial communities when entering soils with animal manure, but understanding the interactions of these effects to the prevailing climatic regime has eluded researchers. A climatic factor that strongly controls microbial activity is soil moisture. Here, we hypothesized that the effects of SDZ on soil microbial communities will be modulated depending on the soil moisture conditions. To test this hypothesis, we performed a 49-day fully controlled climate chamber pot experiments with soil grown withDactylis glomerata(L.). Manure-amended pots without or with SDZ contamination were incubated under a dynamic moisture regime (DMR) with repeated drying and rewetting changes of >20 % maximum water holding capacity (WHCmax) in comparison to a control moisture regime (CMR) at an average soil moisture of 38 % WHCmax. We then monitored changes in SDZ concentration as well as in the phenotypic phospholipid fatty acid and genotypic 16S rRNA gene fragment patterns of the microbial community after 7, 20, 27, 34, and 49 days of incubation. The results showed that strongly changing water supply made SDZ accessible to mild extraction in the short term. As a result, and despite rather small SDZ effects on community structures, the PLFA-derived microbial biomass was suppressed in the SDZ-contaminated DMR soils relative to the CMR ones, indicating that dynamic moisture changes accelerate the susceptibility of the soil microbial community to antibiotics.