High expression of HSP47 in ulcerative colitis-associated carcinomas: proteomic approach.
High expression of HSP47 in ulcerative colitis-associated carcinomas: proteomic approach.
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DOI：
10.1038/sj.bjc.6605163
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发表时间：
2009-08-04
影响因子：
8.8
通讯作者：
Okayasu, I.
中科院分区：
文献类型：
--
作者：
Araki, K.;Mikami, T.;Yoshida, T.;Kikuchi, M.;Sato, Y.;Oh-ishi, M.;Kodera, Y.;Maeda, T.;Okayasu, I.
溃疡性结肠炎（UC）是一种慢性复发性炎症性肠病，已知与结直肠癌发展风险显著增加相关。使用蛋白质组学分析与二维凝胶电泳和质谱，差异表达的蛋白质之间进行了评估UC相关的癌症和散发性结肠癌细胞系。Western blot和免疫组化证实表达。热休克蛋白47 kDa（HSP 47）被确定为一种蛋白质表达更高的UC相关的癌细胞系，和免疫组化检查证实显着更高的水平HSP 47在UC相关的结肠癌比散发性的同行，表达随着肿瘤病变的进展。进一步发现47 kDa的热休克蛋白与I型胶原在细胞质中共表达，并且HSP47和I型胶原都从培养的细胞释放到培养基中。这些结果表明，HSP 47的过度表达是一个独特的特征，UC相关癌与I型胶原合成，具有可能的临床应用。
Ulcerative colitis (UC) is a chronic relapsing inflammatory bowel disease, known to be associated with a markedly increased risk of colorectal carcinoma development. Using proteomic analysis with two-dimensional gel electrophoresis and mass spectrometry, differentially expressed proteins were assessed between UC-associated cancer and sporadic colon cancer cell lines. Western blot and immunostaining were performed for confirming the expression. Heat-shock protein of 47 kDa (HSP47) was identified as one of the proteins expressed more highly in UC-associated cancer cell lines, and an immunohistochemical examination confirmed significantly higher levels of HSP47 in UC-associated colon cancers than in sporadic counterparts, the expression increasing with a progression of neoplastic lesions. Heat-shock protein of 47 kDa was further found to be coexpressed with type I collagen in the cytoplasm, and both HSP47 and type I collagen were released from cultured cells into the culture medium. These results suggest that overexpression of HSP47 is a unique characteristic of UC-associated carcinoma related to type I collagen synthesis, with possible clinical applications.
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影响因子：
3.2
作者：
Fujii, Shigehiko;Katsumata, Daisuke;Fujimori, Takahiro
通讯作者：
Fujimori, Takahiro
影响因子：
3.9
作者：
Sada, M;Igarashi, M;Mitorni, H
通讯作者：
Mitorni, H
影响因子：
2.2
作者：
Tokuyama, Wataru;Mikami, Tetuo;Okayasu, Isao
通讯作者：
Okayasu, Isao
影响因子：
5.7
作者：
Yamashita, K;Yasuda, S;Okayasu, I
通讯作者：
Okayasu, I
DOI：
10.1016/s0006-291x(03)00352-8
发表时间：
2003-04-04
影响因子：
3.1
作者：
Yokota, SI;Kubota, H;Nagata, K
通讯作者：
Nagata, K