Effects of Cryotherapy Applied at Different Temperatures on Inflammatory Pain During the Acute Phase of Arthritis in Rats
Effects of Cryotherapy Applied at Different Temperatures on Inflammatory Pain During the Acute Phase of Arthritis in Rats
复制标题
不同温度冷冻疗法对大鼠关节炎急性期炎症疼痛的影响
DOI：
10.1093/ptj/pzaa211
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发表时间：
2020
期刊：
影响因子：
3.2
通讯作者：
Okita Minoru
中科院分区：
文献类型：
--
作者：
Sasaki Ryo;Sakamoto Junya;Kondo Yasutaka;Oga Satoshi;Takeshita Idumi;Honda Yuichiro;Kataoka Hideki;Origuchi Tomoki;Okita Minoru
目的冷冻疗法治疗急性疼痛的生物学机制仍不清楚。此外，尚不清楚冷冻疗法的有效性是否取决于所施加的温度。本研究旨在阐明冷冻疗法的生物学效应，并考察冷冻疗法在不同温度下对大鼠的治疗效果。方法本研究采用大鼠膝关节关节炎模型进行实验研究。 35只Wistar大鼠被随机分为关节炎组（AR）、关节炎5℃冷冻治疗组（CR-5）、关节炎10℃冷冻治疗组（CR-10）和假关节炎对照组（CON）。通过将高岭土/角叉菜胶的混合物注射到右膝关节来诱发关节炎。从注射后第二天开始，将右膝关节浸入 5°C 或 10°C 的水中，进行 7 天的冷冻疗法。评估关节横径、压痛阈值和疼痛相关行为，持续 7 天。采用免疫组织化学染色法分析注射后8天膝关节中CD68阳性细胞的数量以及脊髓背角降钙素基因相关肽的表达。结果与AR组相比，CR-5和CR-10组在第3天观察到横径、压力痛阈值和疼痛相关行为的改善。 CR-5、CR-10组CD68阳性细胞数及降钙素基因相关肽表达量较AR组显着降低。 CR-5组和CR-10组之间所有结果均无显着差异。结论冷冻疗法可以通过减少滑膜和中枢敏化来减轻炎性疼痛。此外，观察到低于 10°C 冷冻疗法的效果与应用温度无关。冲击冷冻疗法作为炎症急性期疼痛和肿胀管理的物理治疗方式可能是有益的。需要进行转化人体研究来确定治疗炎性疼痛的有效冷冻治疗温度。
ObjectiveThe biological mechanisms of cryotherapy for managing acute pain remain unclear. Additionally, it is unknown whether the effectiveness of cryotherapy depends on the applied temperature. This study aimed to clarify the biological effects of cryotherapy and to examine the therapeutic effects of cryotherapy applied at different temperatures in rats.MethodsThis was an experimental study using a rat knee joint arthritis model. Thirty-five Wistar rats were randomly divided into arthritis (AR), arthritis with 5°C cryotherapy (CR-5), arthritis with 10°C cryotherapy (CR-10), and sham-arthritis control (CON) groups. Arthritis was induced by injecting a mixture of kaolin/carrageenan into the right knee joint. Cryotherapy was applied for 7 days starting the day after injection by immersing the right knee joint in 5°C or 10°C water. Joint transverse diameter, pressure pain threshold, and pain-related behaviors were assessed for 7 days. The number of CD68-positive cells in the knee joint and the expression of calcitonin gene-related peptide in the spinal dorsal horn 8 days after injection were analyzed by immunohistochemical staining.ResultsImprovements in transverse diameter, pressure pain threshold, and pain-related behaviors were observed in the CR-5 and CR-10 groups on the 3rd day compared with the AR group. The number of CD68-positive cells and the expression of calcitonin gene-related peptide in the CR-5 and CR-10 groups were significantly decreased compared with the AR group. There were no significant differences in all results between the CR-5 and CR-10 groups.ConclusionsCryotherapy can ameliorate inflammatory pain through reduction of synovium and central sensitization. Additionally, the effects of cryotherapy lower than 10°C are observed independent of applied temperature.ImpactCryotherapy may be beneficial as a physical therapy modality for pain and swelling management in the acute phase of inflammation. Translational human study is needed to determine the effective cryotherapy temperature for the inflammatory pain.