FINE-STRUCTURE ANALYSIS OF THE DNA-SEQUENCE REQUIREMENTS FOR AUTONOMOUS REPLICATION OF SACCHAROMYCES-CEREVISIAE PLASMIDS
FINE-STRUCTURE ANALYSIS OF THE DNA-SEQUENCE REQUIREMENTS FOR AUTONOMOUS REPLICATION OF SACCHAROMYCES-CEREVISIAE PLASMIDS
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DOI：
10.1128/mcb.6.7.2354
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发表时间：
1986-07-01
影响因子：
5.3
通讯作者：
SMITH, MM
中科院分区：
文献类型：
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作者：
BOUTON, AH;SMITH, MM
一个自主复制的片段ARS位于组蛋白H4基因下游293个碱基对处的拷贝I H3-H4位点。通过缺失分析确定自主复制所需的序列，以包括ARS共有序列和额外的3“-侧翼区。3“-侧翼酵母序列的外部缺失导致复制功能的丧失。然而，中断所需的3“-侧翼域的10个碱基对的连接扫描取代或更大的内部删除并不损害自主复制。因此，复制取决于侧翼染色体结构域，而不是确切的DNA序列。3“-侧翼结构域中所需的酵母序列的范围是可变的，这取决于相邻质粒载体序列的性质。也就是说，当某些载体序列被放置得太靠近ARS共有序列时，它们会阻止复制。这些结果表明，H4 ARS的功能性3“侧翼结构域是特定的DNA或染色质结构或两者兼而有之。
An autonomously replicating segment, ARS, is located 293 base pairs downstream from the histone H4 gene at the copy-I H3-H4 locus. The sequences needed for autonomous replication were defined by deletion analysis to include in ARS consensus seuqence and an additional 3''-flanking region. External deletions into the 3''-flanking yeast sequences resulted in a loss of replication function. However, disruptions of the required 3''-flanking domain by either 10-base-pair linker-scanning substitutions or larger internal deletions did not impair autonomous replication. Thus, replication is dependent upon a flanking chromosome domain, but not an exact DNA sequence. The extent of the yeast sequences required in the 3''-flanking domain is variable depending on the nature of neighboring plasmid vector sequences. That is, there are certain vector sequences that prohibit replication when they are placed too close to the ARS consensus. These results suggest that the functional 3''-flanking domain of the H4 ARS is a specific DNA or chromatin structure or both.