Legionella pneumophilla bacteria in thermal saline bath.
Legionella pneumophilla bacteria in thermal saline bath.
复制标题
热盐水浴中的嗜肺军团菌。
DOI：
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复制
发表时间：
2013
影响因子：
1.7
通讯作者：
E. Lalke
中科院分区：
文献类型：
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作者：
M. Walczak;A. Krawiec;E. Lalke
客观的
该研究旨在确定浴疗法中使用的热盐水中是否可以发现嗜肺军团菌。
方法
水样是从三个热盐水浴中采集的，由热盐水（Cl - Na 型）提供。细菌总数通过荧光显微镜下的直接显微镜计数确定。使用分子FISH方法确定属于不同系统发育群（真细菌、军团菌和嗜肺军团菌）的细菌数量。
结果
整个研究周期中细菌平均总数和真细菌平均数量最高的是浴槽 1。在每个浴槽收集的所有水样中都鉴定出了属于军团菌属的细菌以及嗜肺军团菌。此外，在收集的水样中还鉴定出含有嗜肺军团菌细胞的生物膜。
结论
水中的细菌数量随着浴缸的使用时间而增加。嗜肺军团菌不仅可以在淡水体中成功生长，而且可以在热盐水浴中成功生长。尽管如此，目前还不确定针对淡水体开发的普遍应用的培养方法是否也适用于热盐水浴。
OBJECTIVE
The study was aimed at determining whether Legionella pneumophila bacteria can be found in thermal saline waters used in balneotherapy.
METHODS
Water samples were collected from three thermal saline baths, supplied by thermal saline waters (type Cl - Na). The total number of bacteria was determined in the direct microscopic count under a fluorescence microscope The numbers of bacteria belonging to different phylogenetic groups (Eubacteria, Legionella sp. and Legionella pneumophila) were determined with the use of a molecular FISH method.
RESULTS
The highest average total number of bacteria as well as the highest average number of Eubacteria in the entire research cycle was recorded in bath 1. Bacteria belonging to the Legionella genus along with Legionella pneumophila were identified in all water samples collected from each bath. Moreover, biofilm containing cells of L. pneumophilla was identified in the collected water samples.
CONCLUSION
The number of bacteria in water increases with the bath's age. The Legionella pneumophila can successfully develop not only in fresh water bodies but in thermal saline baths as well. Still, it is uncertain whether the commonly applied culture method, developed for freshwater bodies, is also suitable for thermal saline baths.