Evaluation of serological test of Zika in an endemic area of flavivirus in the Colombian Caribbean
Evaluation of serological test of Zika in an endemic area of flavivirus in the Colombian Caribbean
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DOI：
10.1186/s12941-019-0328-7
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发表时间：
2019-10-14
影响因子：
5.7
通讯作者：
Doria, Amanda
中科院分区：
文献类型：
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作者：
Arrieta, German;Mattar, Salim;Doria, Amanda
寨卡病毒(Zika Virus，ZIKV)是一种新出现的黄病毒，主要通过节肢动物传播，流行于非洲、亚洲和美洲，被世界卫生组织视为全球威胁。目的：评估一种商品化的寨卡病毒检测方法(IgG/IgM目录号B815C，Biocan，Canada.)。方法：对30例登革热、钩端螺旋体、疟疾、汉坦病毒和基孔肯雅患者的血清进行评估。结果：特异性为23.3%(7/30)，急性期为63.6%(7/30)，临床可疑病例为80%(n=10)。两组总的敏感度(IgM)为71.4%(15/21)。结论：该试验特异性较低，不适用于黄病毒流行地区的血清学检测。
The Zika virus (ZIKV) is an emerging flavivirus transmitted primarily through arthropods, endemic in Africa, Asia, and the Americas, and is considered a global threat by the World Health Organization.Objective: To evaluate a commercial Zika virus test (IgG/IgM catalog number B815C, Biocan, Canada.Methods: We evaluated 30 sera of patients diagnosed with Dengue, Leptospira, Malaria, Hantavirus, and Chikungunya. To establish the sensitivity of the test, two groups of sera were analyzed, the first one was patients with Zika RT-qPCR positive, and the second were patients RT-qPCR negative but with clinical suspicion of Zika.Results: The specificity was of 23.3% (7/30), the sensitivity in acute patients with positive RT-qPCR was of 63.6%, the patients with clinical suspicion of Zika the sensitivity (IgM) was of 80% (n = 8/10). Overall sensitivity (IgM) of both groups was of 71.4% (15/21).Conclusions: The test showed a low specificity to be used as a serological test in an endemic area of flavivirus infection.