Toward understanding barnacle cementing by characterization of one cement protein-100kDa in Amphibalanus amphitrite.
Toward understanding barnacle cementing by characterization of one cement protein-100kDa in Amphibalanus amphitrite.
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通过对 Amphibalanus amphitrite 中一种胶结蛋白 100kDa 的表征来了解藤壶胶结。
DOI：
10.1016/j.bbrc.2017.11.101
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发表时间：
2018
影响因子：
3.1
通讯作者：
P. Qian
中科院分区：
文献类型：
--
作者：
Li;Gen Zhang;Yong Wang;Guoyong Yan;P. Qian
藤壶作为主要的污染生物，越来越受到人们的关注。毫无疑问，对水泥蛋白的研究是阐明藤壶胶结机理的必要条件。本研究从amamphibalanus amphitriti中鉴定了一种水泥蛋白Aa-cp100k。Aa-cp100k的氨基酸序列与其他3种藤壶包括rosa megabalanus （Mr-cp100k）、Tetraclita japonica formosana（Tj-cp100k）和policipes policipes （Pp-cp100k）具有高度的相似性。此外，免疫荧光显微镜检测到Aa-cp100k在壶体β分泌细胞和成体骨水泥腺细胞的空泡中定位，表明Aa-cp100k在壶体和成体藤壶中均存在。来自基底的Aa-cp100k可以溶解在尿素缓冲液中，而不需要高浓度的二硫苏糖醇（DTT），这与巨斑玫瑰不同，表明cp100k在胶结中的作用可能是多种多样的。
Barnacles, as major fouling organisms, have attracted more attentions. It is no doubt that the study on cement proteins is required to illustrate the mechanism of barnacle cementing. A cement protein defined as Aa-cp100k was characterized fromAmphibalanus amphitritein this study. The amino acid sequence of Aa-cp100k was shown a high similarity to other three barnacles includingMegabalanus rosa(Mr-cp100k),Tetraclita japonica formosana(Tj-cp100k) andPollicipes pollicipes(Pp-cp100k). Moreover, the localization of Aa-cp100k in the vacuoles of cyprid β secretory cells and the adult cement gland cells by immunofluorescence microscopy, indicating that Aa-cp100k existed in both cyprid and adult barnacle. Aa-cp100k from basal plate could be dissolved in urea buffer without high concentration of dithiothreitol (DTT), different from that inMegabalanus rosa, implying diverse possible roles of cp100k in cementing.
影响因子：
4.1
作者：
Kamino, K
通讯作者：
Kamino, K