Galleria mellonella as an alternative infection model for Yersinia pseudotuberculosis
Galleria mellonella as an alternative infection model for Yersinia pseudotuberculosis
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DOI：
10.1099/mic.0.026823-0
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发表时间：
2009-05-01
期刊：
影响因子：
2.8
通讯作者：
Titball, Richard W.
中科院分区：
文献类型：
--
作者：
Champion, Olivia L.;Cooper, Ian A. M.;Titball, Richard W.
我们报告说，幼虫的蜡螟（大蜡螟）是容易感染的人类肠道病原体假结核耶尔森氏菌在37摄氏度。共聚焦显微镜显示，在感染的初始阶段，细菌被吸收到血细胞中。评价该模型在筛选Y.我们构建并测试了超氧化物歧化酶C（sodC）突变体。该突变体表现出对超氧化物的敏感性增加，这是杀死昆虫血细胞和哺乳动物吞噬细胞的关键机制。在小鼠耶尔森氏菌感染模型中显示出降低的毒力，与野生型菌株IP 32953相反，其不能杀死G.梅隆内拉互补突变体恢复了与SodC相关的所有表型特性，证实了这种金属酶在两种Y.假结核感染模型。
We report that larvae of the wax moth (Galleria mellonella) are susceptible to infection with the human enteropathogen Yersinia pseudotuberculosis at 37 degrees C. Confocal microscopy demonstrated that in the initial stages of infection the bacteria were taken up into haemocytes. To evaluate the utility of this model for screening Y. pseudotuberculosis mutants we constructed and tested a superoxide dismutase C (sodC) mutant. This mutant showed increased susceptibility to superoxide, a key mechanism of killing in insect haemocytes and mammalian phagocytes. It showed reduced virulence in the murine yersiniosis infection model and in contrast to the wildtype strain IP32953 was unable to kill G. mellonella. The complemented mutant regained all phenotypic properties associated with SodC, confirming the important role of this metalloenzyme in two Y. pseudotuberculosis infection models.