K-ras gene mutations in adenomas and carcinomas of the colon.
K-ras gene mutations in adenomas and carcinomas of the colon.
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DOI：
10.1016/0960-7404(92)90088-3
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发表时间：
1992-08-01
期刊：
影响因子：
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通讯作者：
Schofield, P F
中科院分区：
文献类型：
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作者：
Boughdady, I S;Kinsella, A R;Schofield, P F
从29例结直肠癌和40例散发性腺瘤中提取DNA，采用聚合酶链反应（PCR）进行扩增，并使用一组针对正常和突变序列的合成寡核苷酸探针分析K-ras基因密码子12位点突变的存在。突变的存在与各种组织病理学和临床数据相关。10例癌（34.5%）和14例散发性腺瘤（35%）在密码子12处出现K-ras突变。在癌组中，突变癌基因的存在与组织学分化程度、Dukes分期及远处转移的发生无明显相关性。在腺瘤组中，突变的频率随着腺瘤的大小和增生异常变化的严重程度而增加。本研究证实ras基因突变是结肠癌发生的常见和早期事件。它们似乎给那些有突变的息肉提供了选择性生长优势，导致它们的大小增加，从而可能为恶性转化做好准备。
DNA extracted from 29 colorectal carcinomas and 40 sporadic adenomas was amplified by the polymerase chain reaction (PCR) and analysed for the presence of K-ras gene mutations at codon 12 using a panel of synthetic oligonucleotide probes specific for normal and mutated sequences. The presence of mutations was correlated with various histopathological and clinical data. Ten carcinomas (34.5%) and 14 sporadic adenomas (35%) showed K-ras mutations at codon 12. In the carcinoma group, no apparent correlation was found between the presence of mutant oncogenes and the degree of histological differentiation, Dukes' staging or the development of distant metastasis. In the adenoma group, the frequency of mutations increased with the size of the adenoma and the severity of the dysplastic changes. This study confirms that ras gene mutations are common and early events in colon carcinogenesis. They appear to give a selective growth advantage to those polyps with mutations which leads to their increase in size and thus possibly prepare the ground for malignant transformation.