Induction of reactive oxygen intermediates in human monocytes by tumour cells and their role in spontaneous monocyte cytotoxicity
Induction of reactive oxygen intermediates in human monocytes by tumour cells and their role in spontaneous monocyte cytotoxicity
复制标题
肿瘤细胞诱导人单核细胞活性氧中间体及其在自发单核细胞毒性中的作用
作者：
B. Mytar;M. Siedlar;M. Wołoszyn;I. Ruggiero;J. Pryjma;M. Zembala
本研究检测了人单核细胞与肿瘤细胞接触后产生活性氧中间体的能力。单核细胞产生的氧自由基，鲁米诺增强化学发光和超氧阴离子的产生，刺激后与肿瘤，但不与未转化的细胞。使用特定的氧自由基清除剂和抑制剂，超氧化物歧化酶，过氧化氢酶，二甲基亚砜和去铁胺以及髓过氧化物酶抑制剂4-氨基苯甲酸酰肼，表明化学发光依赖于超氧阴离子和羟基自由基的产生和髓过氧化物酶的存在。肿瘤细胞诱导的单核细胞荧光反应显示出不同的动力学与佛波酯激活单核细胞后看到的。这些结果表明，人单核细胞可以直接刺激肿瘤细胞的活性氧中间产物的生产。超氧化物歧化酶、过氧化氢酶、去铁胺和酰肼可抑制单核细胞介导的自发性细胞毒作用，说明超氧阴离子、过氧化氢、羟自由基和次卤酸盐起作用。我们希望表明，所谓的“自发”的新鲜分离的人单核细胞的杀肿瘤能力，实际上可能是一个诱导事件与活性氧中间体的产生，也许是其他有毒介质，从接触的单核细胞与肿瘤细胞。
SummaryThe present study examined the ability of human monocytes to produce reactive oxygen intermediates after a contact with tumour cells. Monocytes generated oxygen radicals, as measured by luminol-enhanced chemiluminescence and superoxide anion production, after stimulation with the tumour, but not with untransformed, cells. The use of specific oxygen radical scavengers and inhibitors, superoxide dismutase, catalase, dimethyl sulphoxide and deferoxamine as well as the myeloperoxidase inhibitor 4-aminobenzoic acid hydrazide, indicated that chemiluminescence was dependent on the production of superoxide anion and hydroxyl radical and the presence of myeloperoxidase. The tumour cell-induced chemiluminescent response of monocytes showed different kinetics from that seen after activation of monocytes with phorbol ester. These results indicate that human monocytes can be directly stimulated by tumour cells for reactive oxygen intermediate production. Spontaneous monocyte-mediated cytotoxicity towards cancer cells was inhibited by superoxide dismutase, catalase, deferoxamine and hydrazide, implicating the role of superoxide anion, hydrogen peroxide, hydroxyl radical and hypohalite. We wish to suggest that so-called ‘spontaneous’ tumoricidal capacity of freshly isolated human monocytes may in fact be an inducible event associated with generation of reactive oxygen intermediates and perhaps other toxic mediators, resulting from a contact of monocytes with tumour cells.
衰老人类单核细胞的抗肿瘤特性。
DOI：
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发表时间：
1995
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
McLachlan,JA;Serkin,CD;Morrey,KM;Bakouche,O
通讯作者：
Bakouche,O
影响因子：
11.2
作者：
T. Utsugi;A. Schroit;J. Connor;C. Bucana;I. Fidler
通讯作者：
T. Utsugi;A. Schroit;J. Connor;C. Bucana;I. Fidler