Extraction of proteins from plant tissues for two-dimensional electrophoresis analysis
Extraction of proteins from plant tissues for two-dimensional electrophoresis analysis
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DOI：
10.1002/elps.200390016
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发表时间：
2003-01-01
期刊：
影响因子：
2.9
通讯作者：
Klose, J
中科院分区：
文献类型：
--
作者：
Giavalisco, P;Nordhoff, E;Klose, J
为了提高植物中双向电泳（2-DE）可检测蛋白质的数量，我们提出了一种从植物组织中提取总蛋白质的新方法。该方法避免了样品制备过程中蛋白质的任何损失，并产生两种不同的级分，一种主要包含细胞质蛋白，另一种主要包含结构键蛋白。从这两个馏分中获得的2-DE图案显示，检测到的不同蛋白质点的总数超过了通常报道的植物蛋白质的分辨率的三倍。
To increase the number of proteins detectable by two-dimensional electrophoresis (2-DE) in plants, we present a new procedure for extracting total proteins from plant tissue. This method avoids any loss of proteins in the course of sample preparation and results in two different fractions, one comprising mainly the cytoplasmatic proteins, the other one containing predominantly structure bond proteins. 2-DE patterns obtained from these two fractions show that the total number of different protein spots detected exceeds the degree of resolution commonly reported for plant proteins threefold.