Molecular cloning and characterization of cDNA sequences coding for rat relaxin
Molecular cloning and characterization of cDNA sequences coding for rat relaxin
复制标题
编码大鼠松弛素的 cDNA 序列的分子克隆和表征
DOI：
10.1038/291127a0
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发表时间：
1981
期刊：
影响因子：
64.8
通讯作者：
Hugh D. Niall
中科院分区：
文献类型：
--
作者：
Peter J. Hudson;John D. Haley;M. Cronk;John Shine;Hugh D. Niall
使用合成的寡核苷酸引物，从卵巢 mRNA 序列的克隆“库”中分离出克隆的 DNA 片段，每个片段都包含大鼠松弛素的完整编码序列。这些克隆的核苷酸序列表明，松弛素是作为前松弛素原分子合成的，具有出乎意料的大的105个氨基酸残基的连接肽。
Using a synthetic oligonucleotide primer, cloned DNA fragments, each containing the entire coding sequence of rat relaxin, have been isolated from a clone ‘bank’ of ovarian mRNA sequences. The nucleotide sequence of these clones demonstrates that relaxin is synthesized as a preprorelaxin molecule with an unexpectedly large connecting peptide of 105 amino acid residues.