Comparisons among two serological tests and microscopic examination for the detection of Theileria annulata in cattle in northern Sudan
Comparisons among two serological tests and microscopic examination for the detection of Theileria annulata in cattle in northern Sudan
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DOI：
10.1016/j.prevetmed.2007.05.008
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发表时间：
2007-10-16
影响因子：
2.6
通讯作者：
El Hussein, A. M.
中科院分区：
文献类型：
--
作者：
Salih, D. A.;Hassan, S. M.;El Hussein, A. M.
我们测试的协议之间的显微镜检查（ME），表面蛋白检测酶联免疫吸附试验（TaSP ELISA）和间接荧光检测（IFA）检测2661自然感染牛从北方苏丹（2001年6月和2002年7月之间收集的样品）的环形泰勒虫。在ME中，我们在364/2661头牛（14%）中检测到吡罗酮，ME和血清学试验之间的kappas较差（TaSP ELISA 10%; IFA 8%）。TaSP ELISA检测到885/2661头牛为阳性，该样本的Rogan-and-Gladen校正真实患病率估计为30%。IFA的相对灵敏度和特异性（与先前验证的TaSP ELISA相比）分别为70.7%和81.8%。(C)2007 Elsevier B. V.保留所有权利。
We tested the agreement between microscopic examination (ME), a surface protein-detecting enzyme-linked immunosorbent assay (TaSP ELISA) and an indirect fluorescent assay (IFA) for detection of Theileria annulata in 2661 naturally infected cattle from northern Sudan (samples collected between June 2001 and July 2002). In the ME, we detected piroplasms in 364/2661 cattle (14%), and the kappas between the ME and the serological tests were poor (TaSP ELISA 10%; IFA 8%). The TaSP ELISA detected 885/2661 cattle as positive, and the Rogan-and-Gladen corrected true prevalence of this sample was estimated to be 30%. The relative sensitivity and specificity of the IFA (compared to the previously validated TaSP ELISA) were 70.7% and 81.8%, respectively. (C) 2007 Elsevier B.V. All rights reserved.