Gene expression of jojoba (Simmondsia chinensis) leaves exposed to drying
Gene expression of jojoba (Simmondsia chinensis) leaves exposed to drying
复制标题
干燥后荷荷巴（Simmondsia chinensis）叶的基因表达
DOI：
10.1016/j.envexpbot.2007.10.026
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发表时间：
2008-05-01
影响因子：
5.7
通讯作者：
Zhang, Genfa
中科院分区：
文献类型：
--
作者：
Geng, Hongwei;Shi, Lu;Zhang, Genfa
以希蒙得木（Simmondsia chinensis（Link）Schnieder）为材料，研究了伤口水分胁迫对荷荷芭基因表达的影响。利用从干燥胁迫下的叶片的受伤部分制备的cDNA作为测试者和从未胁迫的叶片部分制备的cDNA作为驱动者，进行抑制性消减杂交（SSH）。构建了正向消减cDNA文库，经差异筛选得到1344个阳性克隆。测序和修剪后，838个序列进一步分析。序列拼接分析共获得385个独特的EST。通过对NCBI数据库和拟南芥蛋白质功能分类的检索，共获得13大类139条ESTs。使用京都基因和基因组百科全书（KEGG）数据库来评估EST的功能和它们所涉及的途径。通过KEGG Orthology（KO）鉴定器鉴定了96个基因。这些基因涉及63条通路。能量代谢、脂质代谢、氨基酸代谢、蛋白质翻译、MAPK信号通路等途径与伤口水分胁迫有关。本研究结果有助于了解霍霍巴在伤水胁迫下的遗传调控过程。(c)2008 Elsevier B.V.保留所有权利。
Jojoba (Simmondsia chinensis (Link) Schnieder) was used to identify genes regulated by wound-water stress. Suppression subtractive hybridization (SSH) was performed using cDNAs prepared from wounded parts of leaves under drying stress as a tester and cDNAs from unstressed parts of leaves as a driver. A forward-subtracted cDNA library was constructed and positive clones were confirmed by differential screening, resulting in 1344 clones as wound-water stress induced. After sequencing and trimming, 838 sequences were further analyzed. Sequence assembly analysis generated 385 unique ESTs. By refer-ring to NCBI database and the functional categories of Arabidopsis thaliana proteins, 139 ESTs in 13 main categories were annotated. The Kyoto Encyclopedia of Genes and Genomes (KEGG) database was used to evaluate the functions of the ESTs and the pathways in which they are involved. Ninety-six genes were identified by KEGG Orthology (KO) identifier. These genes are involved in 63 pathways. Some pathways, such as energy metabolism, lipid metabolism, amino acid metabolism, translation, and MAPK signaling pathway, are associated with wound-water stress. The results from this study are useful in understanding the genetic regulation process under wound-water stress in jojoba. (c) 2008 Elsevier B.V. All rights reserved.