Production of S-lactoylglutathione by organic-solvent-extracted glyoxalase I from Hansenula mrakii
Production of S-lactoylglutathione by organic-solvent-extracted glyoxalase I from Hansenula mrakii
复制标题
有机溶剂提取汉逊酵母乙二醛酶 I 生产 S-乳酰谷胱甘肽
DOI：
10.1007/bf00170185
复制
发表时间：
2004
影响因子：
5
通讯作者：
A. Kimura
中科院分区：
文献类型：
--
作者：
Y. Inoue;H. Tsuchiyama;N. Kosugi;A. Kimura
用含有50%丙酮(酶活力35个单位/g细胞)或50%乙酸乙酯(酶活力28个单位/g细胞)的缓冲液在30℃下孵育10 h，可从MrakiiHansenula mrakiiIFO 0895细胞中提取乙二酸酶I，但乙二酸酶II在有机溶剂中孵育会失去活性，特别是在较高的温度(30℃)下。用有机溶剂萃取法提取的H。对MrakII的酶促合成乳糖基谷胱甘肽进行了研究，从120mmoL/L(30g/L)酶法制备了约82mmoL/L的乳糖基谷胱甘肽。
Glyoxalase I was extracted fromHansenula mrakiiIFO 0895 by incubating the cells with buffer solution containing 50% acetone (enzyme activity 35 units/g cells) or 50% ethyl acetate (enzyme activity 28 units/g cells) at 30°C for 10 h. Glyoxalase II was also extracted from the cells, although the activity of the enzyme was lost during incubation with organic solvents, especially at higher temperature (30°C). By using the organic-solvent-extracted fraction ofH. mrakii, enzymatic production ofS-lactoylglutathione was studied, and approximately 82 mmol/l (30 g/l) ofS-lactoylglutathione was produced from 120 mmol/l glutathione.