Current status and perspectives of patient-derived xenograft models in cancer research.
Current status and perspectives of patient-derived xenograft models in cancer research.
复制标题
肿瘤研究中患者源性异种移植模型的现状与展望。
DOI：
10.1186/s13045-017-0470-7
复制
发表时间：
2017-05-12
影响因子：
28.5
通讯作者：
Li P
中科院分区：
文献类型：
--
作者：
Lai Y;Wei X;Lin S;Qin L;Cheng L;Li P
癌症仍然是世界范围内的一个主要公共卫生问题，仍然需要在基础和临床前领域进行深入研究。患者来源的异种移植(PDX)模型是将患者原发肿瘤的癌细胞或组织移植到免疫缺陷小鼠体内，模拟体内的人类肿瘤生物学，已广泛应用于癌症研究。植入途径似乎影响了PDX研究的结果，患者来源的原位异种移植(PDOX)模型的应用越来越多。本文首先综述了建立PDX模型的方法学，然后综述了PDX模型在肿瘤生物学特性、起始、增殖、转移和肿瘤微环境等基础研究中的应用和作用，以及在抗癌靶点、药物和治疗策略的临床前探索中的应用，最后对PDX模型的未来前景进行了展望。
Cancers remain a major public health problem worldwide, which still require profound research in both the basic and preclinical fields. Patient-derived xenograft (PDX) models are created when cancerous cells or tissues from patients’ primary tumors are implanted into immunodeficient mice to simulate human tumor biology in vivo, which have been extensively used in cancer research. The routes of implantation appeared to affect the outcome of PDX research, and there has been increasing applications of patient-derived orthotopic xenograft (PDOX) models. In this review, we firstly summarize the methodology to establish PDX models and then go over recent application and function of PDX models in basic cancer research on the areas of cancer characterization, initiation, proliferation, metastasis, and tumor microenvironment and in preclinical explorations of anti-cancer targets, drugs, and therapeutic strategies and finally give our perspectives on the future prospects of PDX models.