Hafnia psychrotolerans sp nov., isolated from lake water
Hafnia psychrotolerans sp nov., isolated from lake water
复制标题
Hafnia psychrotolerans sp nov.，从湖水中分离出来
DOI：
10.1099/ijs.0.000049
复制
发表时间：
2015-03-01
影响因子：
2.8
通讯作者：
Zhang, Shuhong
中科院分区：
文献类型：
--
作者：
Gu, Zhengquan;Liu, Yongqin;Zhang, Shuhong
从青藏高原中国大家错湖分离到一株耐冷、革兰氏阴性、游动、需氧、富含细菌的菌株DJc1-1(T)。该菌株对柠檬酸的利用、脂肪酶活性和α-葡萄糖苷酶为阴性，而对Voges-Proskauer反应和N-乙酰-β-氨基葡萄糖苷酶呈阳性。16S rRNA基因序列分析表明，Hafnia paralvei ATCC 29927(T)、Hafnia alvei ATCC 13337(T)、格里梅氏沙雷氏菌DSM 30063(T)和褶皱沙雷氏菌DSM 4540(T)与DJc1-1(T)的亲缘关系最近，相似性分别为97.76%、96.80%、97.71%和97.58%。菌株DJc1-1(T)的DNA G+C含量为53.9mol%。主要脂肪酸为C-16：0和C-17：0。根据这些特征，菌株DJc1-1(T)可归属于哈夫尼亚属。在DNA DNA杂交试验中，DJc1-1(T)株与模式株副链球菌、链球菌、格氏链球菌和丛枝链球菌的DNA同源性分别为50.6%、35.1%、36.5%和18.1%。生长温度范围为0~40℃，最适生长温度为15℃。生理生化试验表明，DJc1-1(T)菌株与哈夫尼亚属的模式菌株不同。因此，菌株DJc1-1(T)被鉴定为哈夫尼亚属的一个新种，命名为哈夫尼亚耐冷菌。11月是提议的。模式菌株为DJc1-1(T)(=JCM 30077(T)=CGMCC1.12806(T))。
A psychrotolerant, Gram-stain-negative, motile, aerobic, peritrichous bacterium, strain DJC1-1(T), was isolated from Lake Dajiaco, Tibetan Plateau, China. The strain was negative for citrate utilization, lipase activity and alpha-glucosidase, but positive for the Voges-Proskauer reaction and Nacetyl-beta-glucosaminidase. 16S rRNA gene sequence analysis indicated that Hafnia paralvei ATCC 29927(T), Hafnia alvei ATCC 13337(T), Serratia grimesii DSM 30063(T) and Serratia plymuthica DSM 4540(T) were the closest relatives of strain DJC1-1(T), with similarities of 97.76, 96.80, 97.71 and 97.58%, respectively. The DNA G+C content of strain DJC1-1(T) was 53.9 mol%. The predominant fatty acids were C-16:0 and C-17:0 cyclo. Based on these characteristics, strain DJC1-1(T) can be assigned to the genus Hafnia. In DNA DNA hybridization tests, strain DJC1-1(T) shared 50.6, 35.1, 36.5 and 18.1% DNA DNA relatedness with the type strains of H. paralvei, H. alvei, S. grimesii and S. plymuthica, respectively. The growth temperature ranged from 0 to 40 degrees C, with optimum growth at 15 degrees C. Physiological and biochemical tests differentiated strain DJC1-1(T) from the type strains of recognized species of the genus Hafnia. Therefore, strain DJC1-1(T) is identified as representing a novel species of the genus Hafnia, for which the name Hafnia psychrotolerans sp. nov. is proposed. The type strain is DJC1-1(T)(=JCM 30077(T)=CGMCC1.12806(T)).