Microbial characterization during the early habitation of the International Space Station
Microbial characterization during the early habitation of the International Space Station
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DOI：
10.1007/s00248-003-1030-y
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发表时间：
2004-02-01
影响因子：
3.6
通讯作者：
Pierson, DL
中科院分区：
文献类型：
--
作者：
Castro, VA;Thrasher, AN;Pierson, DL
对空气、水和表面样本中的微生物群进行的评估提供了国际空间站（ISS）上微生物特征的基线，以深入了解建造和居住初始阶段的细菌和真菌污染。利用16 S基因测序和rep-PCR，分离出63株细菌进行鉴定和指纹图谱的微生物追踪。在分离和指纹图谱的细菌菌株中，有19种菌株彼此相似。这些分子工具的使用使得能够识别以前使用自动生化分析未识别出的细菌，并提供了几种国际空间站污染物来源的明确指示。通过rep-PCR DNA指纹图谱比较，对测序无法鉴定的菌株（布拉德）、根瘤菌和鞘氨醇单胞菌进行了鉴定。几种甲基杆菌菌株的独特DNA指纹为国际空间站供水污染物的来源提供了明确的指示。在监测期间获得的真菌和细菌数据并不表明当前存在对航天器的微生物危害，也没有任何趋势表明存在潜在的健康风险。以前的航天器环境分析表明，微生物污染将随着时间的推移而增加，需要继续监测。
An evaluation of the microbiota from air, water, and surface samples provided a baseline of microbial characterization onboard the International Space Station (ISS) to gain insight into bacterial and fungal contamination during the initial stages of construction and habitation. Using 16S genetic sequencing and rep-PCR, 63 bacterial strains were isolated for identification and fingerprinted for microbial tracking. Of the bacterial strains that were isolated and fingerprinted, 19 displayed similarity to each other. The use of these molecular tools allowed for the identification of bacteria not previously identified using automated biochemical analysis and provided a clear indication of the source of several ISS contaminants. Strains of Brad),rhizobium and Sphingomonas unable to be identified using sequencing were identified by comparison of rep-PCR DNA fingerprints. Distinct DNA fingerprints for several strains of Methylobacterium provided a clear indication of the source of an ISS water supply contaminant. Fungal and bacterial data acquired during monitoring do not suggest there is a current microbial hazard to the spacecraft, nor does any trend indicate a potential health risk. Previous spacecraft environmental analysis indicated that microbial contamination will increase with time and will require continued surveillance.