The subcutaneous inoculation of pH 6 antigen mutants of Yersinia pestis does not affect virulence and immune response in mice
The subcutaneous inoculation of pH 6 antigen mutants of Yersinia pestis does not affect virulence and immune response in mice
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DOI：
10.1099/jmm.0.005678-0
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发表时间：
2009-01-01
影响因子：
3
通讯作者：
Dentovskaya, Svetlana V.
中科院分区：
文献类型：
--
作者：
Anisimov, Andrey P.;Bakhteeva, Irina V.;Dentovskaya, Svetlana V.
建立了两组鼠疫耶尔森菌的等位基因，包括野生型231和1-1996，其结构亚基或整个操纵子的psaA基因缺失的非极性pH 6(-)突变体，以及恢复了依赖于温度和pH合成粘附毛或组成产生pH 6抗原的菌株。这些突变体是由PSA操纵子的定点突变和随后的反式互补产生的。结果表明，pH 6抗原合成或合成的丧失不影响鼠疫菌的毒力，也不影响皮下接种BALB/c幼鼠或免疫动物的平均存活时间。
Two isogenic sets of Yersinia pestis strains were generated, composed of wild-type strains 231 and 1-1996, their non-polar pH 6(-) mutants with deletions in the psaA gene that codes for its structural subunit or the whole operon, as well as strains with restored ability for temperature- and pH-dependent synthesis of adhesion pili or constitutive production of pH 6 antigen. The mutants were generated by site-directed mutagenesis of the psa operon and subsequent complementation in trans. It was shown that the loss of synthesis or constitutive production of pH 6 antigen did not influence Y. pestis virulence or the average survival time of subcutaneously inoculated BALB/c naive mice or animals immunized with this antigen.