A new paradigm for MAPK: structural interactions of hERK1 with mitochondria in HeLa cells.
A new paradigm for MAPK: structural interactions of hERK1 with mitochondria in HeLa cells.
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DOI：
10.1371/journal.pone.0007541
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发表时间：
2009-10-22
期刊：
影响因子：
3.7
通讯作者：
Jovin TM
中科院分区：
文献类型：
--
作者：
Galli S;Jahn O;Hitt R;Hesse D;Opitz L;Plessmann U;Urlaub H;Poderoso JJ;Jares-Erijman EA;Jovin TM
细胞外信号调节蛋白激酶1和2（ERK 1/2）是MAPK家族的成员，参与细胞反应中刺激物的转导。它们的长期作用是通过促进特定基因的表达来实现的，而更快的反应是通过直接磷酸化位于整个细胞中的下游效应子来实现的。在这项研究中，我们确定，hERK 1易位到HeLa细胞的线粒体后，增殖刺激。在线粒体环境中，hERK 1与（i）至少5种具有转运（即VDAC 1）、信号传导和代谢相关功能的线粒体蛋白;（ii）组蛋白H2 A和H4;以及（iii）其他胞质蛋白物理缔合。这项工作首次表明线粒体中存在多种ERK复合物，从而为评估ERK 1在HeLa细胞中调节细胞信号传导和运输的功能提供了新的视角。
Extracellular signal-regulated protein kinase 1 and 2 (ERK1/2) are members of the MAPK family and participate in the transduction of stimuli in cellular responses. Their long-term actions are accomplished by promoting the expression of specific genes whereas faster responses are achieved by direct phosphorylation of downstream effectors located throughout the cell. In this study we determined that hERK1 translocates to the mitochondria of HeLa cells upon a proliferative stimulus. In the mitochondrial environment, hERK1 physically associates with (i) at least 5 mitochondrial proteins with functions related to transport (i.e. VDAC1), signalling, and metabolism; (ii) histones H2A and H4; and (iii) other cytosolic proteins. This work indicates for the first time the presence of diverse ERK-complexes in mitochondria and thus provides a new perspective for assessing the functions of ERK1 in the regulation of cellular signalling and trafficking in HeLa cells.