KIN RECOGNITION BETWEEN MEDIAL GOLGI ENZYMES IN HELA-CELLS
KIN RECOGNITION BETWEEN MEDIAL GOLGI ENZYMES IN HELA-CELLS
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DOI：
10.1002/j.1460-2075.1994.tb06294.x
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发表时间：
1994-02-01
期刊：
影响因子：
11.4
通讯作者：
WARREN, G
中科院分区：
文献类型：
--
作者：
NILSSON, T;HOE, MH;WARREN, G
中间高尔基体酶N-乙酰氨基葡萄糖基转移酶I(NAGT I)和甘露糖苷酶II(Mann II)和反式高尔基体酶β-1，4-半乳糖基转移酶(GALT)通过嫁接在p33不变链的胞质尾部保留在内质网(ER)中。P33/NAGT I在HeLa细胞中的瞬时稳定表达导致内源性Mann II向内质网迁移，p33/Mann II的瞬时表达对内源性NAGT I也有类似的影响，p33/GALT的瞬时表达不影响这两种内源性中间酶。这些数据为中间高尔基酶之间的亲缘识别提供了强有力的证据，并表明它们在高尔基堆栈的组织中发挥了作用。
The medial Golgi enzymes, N-acetylglucosaminyltransferase I (NAGT I) and mannosidase II (Mann II), and the trans Golgi enzyme, beta-1,4-galactosyltransferase (GalT) were each retained in the endoplasmic reticulum (ER) by grafting on the cytoplasmic tail of the p33 invariant chain. Transient and stable expression of p33/NAGT I in HeLa cells caused relocation of endogenous Mann II to the ER and transient expression of p33/Mann II had a similar effect on endogenous NAGT I. Neither of these endogenous medial enzymes were affected by transient expression of p33/GalT. These data provide strong evidence for kin recognition between medial Golgi enzymes and suggest a role for them in the organization of the Golgi stack.