Molecular dissection of early stages of the eukaryotic secretory pathway
Molecular dissection of early stages of the eukaryotic secretory pathway
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DOI：
10.1016/0955-0674(90)90104-m
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发表时间：
1990-08-01
影响因子：
7.5
通讯作者：
Balch, W. E.
中科院分区：
文献类型：
--
作者：
Balch, W. E.
真核细胞分泌途径的所有区室之间的蛋白质转运由载体囊泡的顺序形成（出芽）和融合控制。这些囊泡控制内质网 (ER)、高尔基体和细胞表面之间蛋白质的组成和调节流动。随着用于研究分泌途径每个阶段的部分反应的体外模型系统的出现，以及用于剖析酵母途径的遗传系统的发展，囊泡出芽和靶向的生化和分子基础，以及囊泡-膜融合的基本机制已成为密切关注的主题。由于最近对囊泡转运的基本特性进行了综述[1, 2]，因此本讨论将仅集中于我们对分泌途径早期区室（ER 和 Go@）之间蛋白质转运的生化和分子基础的理解的新进展。
Transport of protein between all compartments of the secretory pathway of eukaryotic cells is governed by the sequential formation (budding) and fusion of carrier vesicles. These vesicles control both the constitutive and regulated flow of protein between the endoplasmic reticulum (ER), the Golgi and the cell surface. The biochemical and molecular basis for vesicle budding and targeting, and the mechanisms fundamental to vesicle-membrane fusion have become the subject of intense scrutiny with the advent of model in vitro systems to study these partial reactions for each stage of the secretory pathway, and the development of a genetic system for dissecting the pathway in yeast. As the basic properties of vesicular transport have been recently reviewed [1, 2], the present discussion will focus only on new developments in our understanding of the biochemical and molecular basis for transport of protein between the early compartments (ER and Go@) of the secretory pathway.