LRG1 promotes corneal angiogenesis and lymphangiogenesis in a corneal alkali burn mouse model
LRG1 promotes corneal angiogenesis and lymphangiogenesis in a corneal alkali burn mouse model
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LRG1促进角膜碱烧伤小鼠模型中的角膜血管生成和淋巴管生成
DOI：
10.18240/ijo.2020.03.01
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发表时间：
2020-03-18
影响因子：
1.4
通讯作者：
Yang, Ling-Ling
中科院分区：
文献类型：
--
作者：
Song, Shan;Cheng, Jun;Yang, Ling-Ling
目的：探讨富亮氨酸α -2糖蛋白-1 （LRG1）对角膜血管生成和淋巴管生成的潜在作用及其机制。方法：建立碱烧伤小鼠模型，诱导角膜新生血管和淋巴管形成。免疫荧光染色检测LRG1在角膜组织中的位置，验证LRG1阳性细胞的来源。局部应用外源性LRG1蛋白或LRG1 siRNA后，对角膜进行CD31（一种泛内皮细胞标志物）和淋巴内皮羟酸受体-1 （LYVE-1；淋巴标志物）的全贴壁染色，检测血液和淋巴管的生长情况。此外，采用Western blot方法检测促血管生成性血管内皮生长因子（VEGF）相关蛋白的表达。结果：碱烧伤角膜缘区和中心区角膜基质中LRG1显著升高。角膜基质中lrg1阳性细胞主要来源于vimentin阳性细胞。局部应用外源LRG1蛋白不仅能显著促进血管生成，还能显著促进淋巴管生成(P
AIM: To investigate the potential effect and mechanism of leucine-rich alpha-2-glycoprotein-1 (LRG1) on corneal angiogenesis and lymphangiogenesis.METHODS: Corneal neovascularization and lymphatics were induced by establishing alkali burn mouse model. Immunofluorescence staining was performed to detect the location of LRG1 in cornea tissues and to verify the source of LRG1-positive cells. Corneal whole-mount staining for CD31 (a panendothelial cell marker) and lymphatic endothelial hyluronan receptor-1 (LYVE-1; lymphatic marker) was performed to detect the growth of blood and lymphatic vessels after local application of exogenous LRG1 protein or LRG1 siRNA. In addition, expressions of the proangiogenic vascular endothelial growth factor (VEGF) related proteins were detected using Western blot analysis.RESULTS: LRG1 was dramatically increased in alkali burned corneal stroma in both the limbal and central areas. LRG1-positive cells in the corneal stroma were mainly derived from Vimentin-positive cells. Local application of exogenous LRG1 protein not only aggravated angiogenesis but also lymphangiogenesis significantly (P