Effects of anesthetics on expression of dopamine and acetylcholine receptors in the rat brain in vivo
Effects of anesthetics on expression of dopamine and acetylcholine receptors in the rat brain in vivo
复制标题
DOI：
10.1007/s00540-022-03046-7
复制
发表时间：
2022-02
影响因子：
2.8
通讯作者：
Keisuke Yoshida;M. Murakawa;Atsuyuki Hosono
中科院分区：
文献类型：
--
作者：
Keisuke Yoshida;M. Murakawa;Atsuyuki Hosono
多巴胺D2和乙酰胆碱M1受体可能与术后认知功能障碍有关。本研究的目的是探讨几种用于全身麻醉和/或镇静的麻醉药是否影响大鼠脑内多巴胺D2和乙酰胆碱M1受体的表达。将36只5-9周龄雄性大鼠分为6组（每组n= 6）; 5组为麻醉剂组，1组为对照组。5组分别用右美托咪定0.4 µg/kg/min、丙泊酚50 mg/kg/h、咪达唑仑25 mg/kg/h、七氟烷3.3%或氧化亚氮75%麻醉4 h。然后，将大鼠断头，并从所有大鼠收集大脑皮质、海马、纹状体、脑干和小脑。实时荧光定量PCR检测脊髓多巴胺D2受体（Drd 2）和脊髓乙酰胆碱M1受体（Chrm 1）的表达。各组脑内各区域Drd 2和Chrm 1 mRNA表达量无显著性差异。在本研究中使用的每种麻醉剂剂量下，不太可能发生右美托咪定、丙泊酚、咪达唑仑、七氟烷和一氧化二氮给药引起的多巴胺D2和乙酰胆碱M1受体突触后变化。
Dopamine D2and acetylcholine M1receptors might be related to post-operative cognitive dysfunction. The aim of the present study is to investigate whether several anesthetics which are used for general anesthesia and/or sedation, affect expression of dopamine D2and acetylcholine M1receptors in the rat brain. Thirty-six male rats aged 5–9 weeks old were divided into six groups (n= 6 in each group); five groups for anesthetics and one for control. The five groups were anesthetized with either dexmedetomidine 0.4 µg/kg/min, propofol 50 mg/kg/h, midazolam 25 mg/kg/h, sevoflurane 3.3%, or nitrous oxide 75% for 4 h. Then, the rats were decapitated, and the cerebral cortex, hippocampus, corpus striatum, brain stem, and cerebellum were collected from all rats. Then, real-time polymerase chain reaction was performed to examine the expression ofDrd2(cord dopamine D2receptor) andChrm1(cord acetylcholine M1receptor). There were no significant differences among the groups regardingDrd2andChrm1mRNA expression of each region of the brain. Postsynaptic changes of dopamine D2and acetylcholine M1receptors due to administration of dexmedetomidine, propofol, midazolam, sevoflurane, and nitrous oxide are unlikely to occur at the doses of each anesthetic used in the present study.