A conserved role for non-neural ectoderm cells in early neural development
A conserved role for non-neural ectoderm cells in early neural development
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DOI：
10.1242/dev.107425
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发表时间：
2014-11-01
期刊：
影响因子：
4.6
通讯作者：
Camus, Anne
中科院分区：
文献类型：
--
作者：
Cajal, Marieke;Creuzet, Sophie E.;Camus, Anne
在头部发育的早期阶段，外胚层模式导致不同的非神经和神经祖细胞的出现。前板外胚层和神经嵴的诱导取决于注定要成为表皮的非神经外胚层和未来神经板之间经过充分研究的信号相互作用。相比之下，非神经外胚层参与导致中枢神经系统发育和模式形成的形态发生事件的研究较少。在这里，我们发现，在小鼠和鸡胚胎中，在原肠胚形成晚期，去除毗邻预期神经板的吻端非神经外胚层会导致前脑和颅面组织的形态缺陷。特别是，这种消融会损害端脑的发育，但不会影响间脑的发育。对消融小鼠胚胎的进一步研究表明，对前脑区域化至关重要的信号中心，即轴向中内胚层和前神经脊，正常形成。此外，细胞死亡或细胞增殖的变化无法解释端脑组织的特定损失。最后，我们提供的证据表明，头端组织的去除会引发 BMP、WNT 和 FGF 信号通路的失调，从而可能影响端脑的发育。这项研究为神经/非神经界面的作用开辟了新的视角，并揭示了其在高等脊椎动物中的功能相关性。
During the early steps of head development, ectodermal patterning leads to the emergence of distinct non-neural and neural progenitor cells. The induction of the preplacodal ectoderm and the neural crest depends on well-studied signalling interactions between the non-neural ectoderm fated to become epidermis and the prospective neural plate. By contrast, the involvement of the non-neural ectoderm in the morphogenetic events leading to the development and patterning of the central nervous system has been studied less extensively. Here, we show that the removal of the rostral non-neural ectoderm abutting the prospective neural plate at late gastrulation stage leads, in mouse and chick embryos, to morphological defects in forebrain and craniofacial tissues. In particular, this ablation compromises the development of the telencephalon without affecting that of the diencephalon. Further investigations of ablated mouse embryos established that signalling centres crucial for forebrain regionalization, namely the axial mesendoderm and the anterior neural ridge, form normally. Moreover, changes in cell death or cell proliferation could not explain the specific loss of telencephalic tissue. Finally, we provide evidence that the removal of rostral tissues triggers misregulation of the BMP, WNT and FGF signalling pathways that may affect telencephalon development. This study opens new perspectives on the role of the neural/non-neural interface and reveals its functional relevance across higher vertebrates.