Molecular characteristics of strains of the Cameroon family, the major group of Mycobacterium tuberculosis in a country with a high prevalence of tuberculosis
Molecular characteristics of strains of the Cameroon family, the major group of Mycobacterium tuberculosis in a country with a high prevalence of tuberculosis
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DOI：
10.1128/jcm.42.11.5029-5035.2004
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发表时间：
2004-11-01
影响因子：
9.4
通讯作者：
Gutierrez, MC
中科院分区：
文献类型：
--
作者：
Niobe-Eyangoh, SN;Kuaban, C;Gutierrez, MC
在结核病高发国家喀麦隆，对结核分枝杆菌复合菌株遗传多样性的初步调查描述了一组密切相关的结核分枝杆菌菌株(喀麦隆家族)，目前导致40%以上的涂阳肺结核病例。在这里，我们使用各种分子方法来研究这一家族菌株的遗传特征。喀麦隆结核分枝杆菌菌株(I)是主要基因群2的一部分，像其他流行菌株家族一样缺乏Tbd1区，(Ii)在其直接重复(DR)区缺少间隔区23、24和25，(Iii)在分枝杆菌散布重复单元(Miru-VNTR)的12个可变数目串联重复序列(Miru-VNTR)基因座中有8个具有相同数目的重复数，(Iv)具有类似的IS6110-限制性片段长度多态性(RFLP)多带图谱(10至15个拷贝)，具有7个共同的IS6110条带，(V)在其DR基因座上没有IS6110元素，和(Vi)在开放阅读框中有四个IS6110元件(腺苷环化酶、磷脂酶C、Moey和ATP结合基因)。通过SPOGO分型、Miru-VNTR和IS6110-RFLP分型方法的分析发现了以前研究中没有观察到的差异；Miru-VNTR分型评估的多态低于SPOGO分型的建议，在极少数情况下，具有相同IS6110-RFLP模式的菌株具有多达15个间隔区的SP寡型差异。我们的发现证实了该家族最近在喀麦隆的扩大，并表明对分子分型结果的解释必须适应每个环境中菌株群体的特征。由于这种特殊的基因型别与喀麦隆的结核病有很大关系，因此可以通过解释这一地理区域的数据来更好地进行结核病传播研究。
A preliminary investigation of the genetic biodiversity of Mycobacterium tuberculosis complex strains in Cameroon, a country with a high prevalence of tuberculosis, described a group of closely related M. tuberculosis strains (the Cameroon family) currently responsible for more than 40% of smear-positive pulmonary tuberculosis cases. Here, we used various molecular methods to study the genetic characteristics of this family of strains. Cameroon family M. tuberculosis strains (i) are part of the major genetic group 2 and lack the TbD1 region like other families of epidemic strains, (ii) lack spacers 23, 24, and 25 in their direct repeat (DR) region, (iii) have an identical number of repeats in 8 of 12 variable-number tandem repeats of mycobacterial interspersed repetitive unit (MIRU-VNTR) loci, (iv) have similar IS6110-restriction fragment length polymorphism (RFLP) multiband patterns (10 to 15 copies) with seven common IS6110 bands, (v) do not have an IS6110 element in their DR locus, and (vi) have four IS6110 elements in open reading frames (adenylate cyclase, phospholipase C, moeY, and ATP binding genes). Analysis by spoligotyping, MIRU-VNTR, and IS6110-RFLP typing methods revealed differences not observed in previous studies; polymorphism as assessed by MIRU-VNTR typing was lower than suggested by spoligotyping, and in rare cases, strains with identical IS6110-RFLP patterns had spoligotypes differing by as much as 15 spacers. Our findings confirm the recent expansion of this family in Cameroon and indicate that the interpretation of molecular typing results has to be adapted to the characteristics of the strain population within each setting. The knowledge of this particular genotype, with its large involvement in tuberculosis in Cameroon, allows greater refinement of tuberculosis transmission studies by interpreting data in the context of this geographic area.