Unique intravascular tumor microenvironment predicting recurrence of lung squamous cell carcinoma
Unique intravascular tumor microenvironment predicting recurrence of lung squamous cell carcinoma
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DOI：
10.1007/s00432-015-2068-1
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发表时间：
2016-03-01
影响因子：
3.6
通讯作者：
Ishii, Genichiro
中科院分区：
文献类型：
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作者：
Hisakane, Kakeru;Saruwatari, Koichi;Ishii, Genichiro
目的肿瘤的组织学血管侵犯（VI）是手术切除后复发的危险因素。然而，VI 特征在组织学上有所不同。本研究的目的是确定与肺鳞状细胞癌 (SCC) 复发相关的特征性 VI 特征。方法 我们在本研究中纳入了 149 名病理学 I 期原发性肺 SCC 患者，并检查 VI 的存在、频率和大小是否与复发相关。我们还评估了 VI 区域内癌细胞和基质细胞的免疫表型。结果 在 149 名患者中，58 名患有 VI 肿瘤。 VI 的存在与较短的无复发生存期 (RFS) 显着相关 (P = 0.018)。虽然 VI 频率与 RFS 无关，但较大的 VI 尺寸 (> 425 A mu m) 与较短的 RFS 显着相关 (P = 0.003)。较大 VI 区域内的癌细胞表达的平足蛋白（癌症干细胞标记物）水平显着升高 (P = 0.039)；与较小的 VI 区域相比，较大的 VI 区域内存在更多数量的 CD34(+) 微血管 (P = 0.009)、CD204(+) 巨噬细胞 (P = 0.026) 和 α-SMA(+) 肌成纤维细胞 (P = 0.056)。此外，在较大的血管内，癌症干细胞和丰富的基质细胞可以为肿瘤转移创造更有利的微环境。
Purpose Histological vascular invasion (VI) by tumors is a risk factor for recurrence after surgical resection. However, VI features vary histologically. The aim of this study was to identify characteristic VI features that are associated with recurrence in squamous cell carcinoma (SCC) of the lung. Methods We enrolled 149 patients with pathological stage I primary lung SCC in this study and examined whether the presence, frequency, and size of VI were associated with recurrence. We also evaluated immunophenotypes of carcinoma cells and stromal cells within VI areas.Results Of the 149 patients, 58 had tumors with VI. The presence of VI was significantly correlated with shorter recurrence-free survival (RFS) (P = 0.018). Although VI frequency was not associated with RFS, larger VI size (> 425 A mu m) was significantly correlated with shorter RFS (P = 0.003). Carcinoma cells within larger VI areas expressed significantly higher levels of podoplanin, cancer stem cell marker (P = 0.039); higher numbers of CD34(+) microvessels (P = 0.009), CD204(+) macrophages (P = 0.026), and alpha-SMA(+) myofibroblasts (P = 0.056) were present within larger VI areas than within smaller VI ones.Conclusions Our results indicate that larger VI areas are a predictor for recurrence in lung SCC; also, within the larger blood vessel, cancer stem cells and abundant stromal cells can create a more favorable microenvironment for tumor metastasis.