Modulation of Calcium Oxalate Crystal Growth and Protection from Oxidatively Damaged Renal Epithelial Cells of Corn Silk Polysaccharides with Different Molecular Weights
Modulation of Calcium Oxalate Crystal Growth and Protection from Oxidatively Damaged Renal Epithelial Cells of Corn Silk Polysaccharides with Different Molecular Weights
复制标题
不同分子量玉米须多糖对草酸钙晶体生长的调节及对肾上皮细胞氧化损伤的保护
DOI：
10.1155/2020/6982948
复制
发表时间：
2020-01-22
影响因子：
--
通讯作者：
Ouyang, Jian-Ming
中科院分区：
文献类型：
--
作者：
Chen, Jia-Yun;Sun, Xin-Yuan;Ouyang, Jian-Ming
玉米须多糖（CSP 0;分子量= 124 kDa）通过超声处理降解以获得5种降解多糖，即CSP 1、CSP 2、CSP 3、CSP 4和CSP 5，分子量分别为26.1、12.2、6.0、3.5和2.0 kDa。通过FT-IR、1H NMR和13 C NMR对多糖的结构进行了表征。在6.0 ~ 124 kDa范围内，随着CSP分子量的减小，CSP的抗氧化活性（包括清除羟自由基和DPPH自由基的能力、螯合铁离子的能力和还原能力）逐渐增强。然而，抗氧化活性减弱，当CSP的分子量达到3.5和2 kDa。分子量为6.0 kDa的CSP 3具有最强的抗氧化活性。经60μg/mL CSP保护后，纳米COM晶体损伤的人近端肾小管上皮细胞（HK-2）活力增加，活性氧水平降低，细胞表面粘附的COM晶体数量减少。CSP保护细胞免受CaOx晶体损伤的能力与其抗氧化活性一致。CSP可以特异性地与CaOx晶体结合联合收割机，以抑制二水草酸钙晶体向一水草酸钙晶体的转化。这些结果表明，CSPs的活性与分子量密切相关。分子量过高或过低的CSP均不利于其活性的发挥。CSPs，特别是分子量为6.0 kDa的CSP 3，可用作潜在的抗结石药物。
Corn silk polysaccharide (CSP0; molecular weight = 124 kDa) was degraded by ultrasonication to obtain five degraded polysaccharides, namely, CSP1, CSP2, CSP3, CSP4, and CSP5, with molecular weights of 26.1, 12.2, 6.0, 3.5, and 2.0 kDa, respectively. The structures of these polysaccharides were characterized by FT‐IR,1H NMR, and13C NMR analyses. The antioxidant activities, including scavenging ability for hydroxyl radicals and DPPH free radicals, chelation ability for Fe2+ions, and reducing ability of CSP increased with decreased molecular weight of CSPs within 6.0 to 124 kDa. However, antioxidant activity weakened when the molecular weight of CSPs reached 3.5 and 2 kDa. CSP3 with a molecular weight of 6.0 kDa exhibited the strongest antioxidant activity. After protection with 60μg/mL CSPs, the viability of human renal proximal tubular epithelial cells (HK‐2) damaged by nano‐COM crystals increased, the level of reactive oxygen species decreased, and the amount of COM crystal adhered onto the cell surface decreased. The ability of CSPs to protect cells from CaOx crystal damage was consistent with their antioxidant activity. CSPs can specifically combine with CaOx crystal to inhibit the conversion of calcium oxalate dihydrate crystal to calcium oxalate monohydrate crystal. All these results showed that the activity of CSPs was closely correlated with molecular weight. A very high or low molecular weight of CSPs was not conducive to their activity. CSPs, especially CSP3 with a molecular weight of 6.0 kDa, can be used as a potential antistone drug.