USE OF MULTICELL SPHEROIDS OF OVARIAN-CARCINOMA AS AN INTRAPERITONEAL RADIO-IMMUNOTHERAPY MODEL - UPTAKE, RETENTION KINETICS AND DOSIMETRIC EVALUATION
USE OF MULTICELL SPHEROIDS OF OVARIAN-CARCINOMA AS AN INTRAPERITONEAL RADIO-IMMUNOTHERAPY MODEL - UPTAKE, RETENTION KINETICS AND DOSIMETRIC EVALUATION
复制标题
DOI：
10.1002/ijc.2910500627
复制
发表时间：
1992-04-01
影响因子：
6.4
通讯作者：
CHATAL, JF
中科院分区：
文献类型：
--
作者：
BARDIES, M;THEDREZ, P;CHATAL, JF
本研究使用多细胞球体作为卵巢癌微转移的体外模型，用于i.p.放射免疫治疗，目的是测量in -111标记的OC125单克隆抗体（MAb）的F(ab')2片段在NIH:OVCAR-3细胞系球体中的摄取和保留动力学，并估计β -放射核素的吸收剂量（动力学假设与铟-111相似）。在不同MAb浓度下，直径为0.2 mm的球体的结合值最高。保留动力学显示生物半衰期为50小时。这些数据被用来通过对伯杰最新点核的积分来计算吸收剂量。被认为在表面（无穿透）或整个球体（总穿透）空间分布均匀时，Sm-153的平均吸收剂量分别为247和417 Gy， Y-90的平均吸收剂量为90和135 Gy。因此，在患者中使用相似的单抗浓度和特异性活性应该导致微转移的i.p.放射免疫治疗的高吸收剂量。
The purpose of this study, using multi-cell spheroids as an in vitro model of micrometastases of ovarian carcinoma for i.p. radio-immunotherapy, was to measure the uptake and retention kinetics of In-111-labeled F(ab')2 fragments of OC125 monoclonal antibody (MAb) in spheroids of the NIH:OVCAR-3 cell line and to estimate absorbed doses with beta-emitting radionuclides (kinetics was assumed to be similar to that of indium-111). With 0.2-mm-diameter spheroids at different MAb concentrations the highest binding value was determined. Retention kinetics showed a biological half-life of 50 hr. These data were used to calculate absorbed doses by integration of Berger's latest-point kernels. Mean absorbed doses when spatial distribution was considered to be uniform at the surface (no penetration) or throughout the spheroid (total penetration) were, respectively, 247 and 417 Gy with Sm-153, and 90 and 135 Gy with Y-90. Thus, the use of a similar MAb concentration and specific activity in patients should lead to high absorbed doses in i.p. radio-immunotherapy of micrometastases.