Hypophysectomy eliminates and growth hormone (GH) maintains the midpregnancy elevation in GH receptor and serum binding protein in the mouse.
Hypophysectomy eliminates and growth hormone (GH) maintains the midpregnancy elevation in GH receptor and serum binding protein in the mouse.
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垂体切除术消除了小鼠妊娠中期 GH 受体和血清结合蛋白的升高，而生长激素 (GH) 维持了这一水平。
作者：
F. Sánchez;P. Fielder;Ricardo R. Martinez;W. Smith;F. Talamantes
在垂体切除妊娠小鼠、假手术妊娠小鼠、完整妊娠小鼠和完整成年处女小鼠中，测定了[125 I]Iodomouse GH [（125 I）iodo-mGH]与血清和肝微粒体膜样品的结合。在妊娠第11天进行剖腹产，并在第14天处死动物。完整的处女小鼠的血清和肝微粒体膜的结合mGH是远远低于那些完整的妊娠小鼠。在垂体切除的小鼠中，血清和肝微粒体的mGH结合能力均降低至与未妊娠小鼠相似的值。在母体血清mGH浓度、血清GH结合蛋白浓度或肝脏GH受体浓度方面，未观察到完整和假手术妊娠动物之间存在显著差异。GH受体和结合蛋白编码的mRNA也高于完整的和假手术妊娠小鼠比处女和垂体切除小鼠。垂体切除小鼠用200微克/天牛GH治疗，通过渗透微型泵给药;治疗3天后，观察到肝脏GH受体和血清GH结合蛋白水平显著升高。这些结果表明，上调肝脏GH受体和血清GH结合蛋白的GH在怀孕期间的小鼠。
[125I]Iodomouse GH [( 125I]iodo-mGH) binding to samples of serum and hepatic microsomal membranes was measured in hypophysectomized pregnant, sham-operated pregnant, intact pregnant, and intact adult virgin mice. Surgeries were carried out on day 11 of pregnancy, and the animals were killed on day 14. The binding of mGH to both serum and hepatic microsomal membranes of intact virgin mice was much lower than to those of intact pregnant mice. In hypophysectomized mice, the mGH-binding capacity of both serum and hepatic microsomes decreased to values similar to those of nonpregnant mice. No significant differences were observed between intact and sham-operated pregnant animals in the maternal serum mGH concentration, the serum GH-binding protein concentration, or the hepatic GH receptor concentration. GH receptor and binding protein-encoding mRNAs were also higher in intact and sham-operated pregnant mice than in virgin and hypophysectomized mice. Hypophysectomized mice were treated with 200 micrograms/day bovine GH, administered by osmotic minipump; after 3 days of treatment, a significant elevation of hepatic GH receptor and serum GH-binding protein levels was observed. These results demonstrate an up-regulation of hepatic GH receptors and serum GH-binding protein by GH during pregnancy in the mouse.
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垂体切除术对小鼠血清胎盘催乳素和孕酮的影响。
影响因子：
4.8
作者：
Day,JR;Ogren,LM;Talamantes,F
通讯作者：
Talamantes,F
DOI：
10.1073/pnas.79.3.771
发表时间：
1982
影响因子：
11.1
作者：
Colosi,P;Marr,G;Lopez,J;Haro,L;Ogren,L;Talamantes,F
通讯作者：
Talamantes,F
影响因子：
4.8
作者：
MAITER, D;UNDERWOOD, LE;KETELSLEGERS, JM
通讯作者：
KETELSLEGERS, JM
小鼠肝膜中生长激素受体异质群体的鉴定和表征。
DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Smith,WC;Talamantes,F
通讯作者：
Talamantes,F
生长激素在大鼠脂肪细胞中维持其自身的受体。
影响因子：
4.8
作者：
Grichting,G;Goodman,HM
通讯作者：
Goodman,HM