A Nanosecond Photon-Counting Fluorimetric System Using a Modified Multichannel Vernier Chronotron
A Nanosecond Photon-Counting Fluorimetric System Using a Modified Multichannel Vernier Chronotron
复制标题
使用改进的多通道 Vernier Chronotron 的纳秒光子计数荧光系统
DOI：
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发表时间：
1985
期刊：
影响因子：
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通讯作者：
S. Minami
中科院分区：
文献类型：
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作者：
T. Iwata;T. Uchida;S. Minami
介绍了一种新型的高数据采集效率的时间分辨光子计数仪。其工作原理是基于测量在短时间的瞬时发射期间发射的光子爆发的纳秒时间分布。该仪器的特点是具有改进型游标计时器的独特功能，具有多个符合电路和串行定时数据存储器，可用作1.5 ns时间分辨率的高效多通道事件时间分析器。与传统的单光子计数法相比，数据采集效率提高了20倍以上。在正常运行时，得到了144 ns周期内的瞬时发射的时间历程；对于持续时间较长的现象，给出了运行的时间尺度展开模式。为了验证整个体系的性能，给出了1-ppm硫酸奎宁在0.1-N硫酸溶液中的荧光衰减曲线。
A new time-resolved photon-counting instrument with high data-gathering efficiency is described. The principle of operation is based on the measurement of the nanosecond temporal distribution of the emitted photon burst during the short duration of transient emission. The instrument is characterized by the unique capabilities of a modified vernier chronotron with plural coincidence circuits and serial-timing data memories, which serve as an efficient multichannel event-time analyzer of 1.5-ns time resolution. The data-gathering efficiency is improved by a factor of 20 or more in comparison with that of the conventional single-photon counting method. In regular operation, the time history of transient emission for the period of 144 ns is obtained; and for phenomena with longer duration, the time-scale expansion mode of operation is provided. To demonstrate the whole system performance, a fluorescence decay curve of 1-ppm quinine sulfate in 0.1-N H2SO4 is presented.